IMMUNOPATHOLOGY OF HIV-1 VPR

描述(由申请人提供)：本项目的中心目标是 了解vpr在HIV-1免疫发病机制和疾病中的作用 进步。申请人建议评估VPR调解的机制 调节CD4T细胞和巨噬细胞的反应，并评估 VPR调节体内对病毒感染的免疫反应的可能性。 具体来说，研究小组将：(1)调查VPR的直接影响 在体外对抗原提呈细胞和T细胞的影响；(2)测定 其中VPR使用体外免疫模型来调节免疫失调； (3)确定参与调节这些免疫功能的vpr结构域 使用VPR突变体。拟议的研究将导致对如何 VPR调节这些重要免疫细胞类型的基本细胞事件，并 它如何在体外和体内调节免疫功能。

DESCRIPTION (provided by applicant): The central objective of this project is to understand the role of Vpr in HIV-1 immunopathogenesis and disease progression. The Applicant proposes to evaluate the mechanisms of Vpr-mediated regulation of CD4+ T cell and macrophage responses, and to assess the possibility that Vpr modulates the in vivo immune response to viral infection. Specifically, the research team will: (1) investigate the direct effects of Vpr on antigen presenting cells and T cells in vitro; (2) determine the extent to which Vpr mediates immune dysregulation using an in vitro immunization model; (3) identify the domains of Vpr involved in mediating these immune functions using Vpr mutants. The proposed studies will lead to an understanding of how Vpr modulates basic cellular events in these important immune cell types and how it may regulate immune functions in vitro and in vivo.