IMMUNOPATHOLOGY OF HIV-1 VPR

描述（由申请人提供）：该项目的中心目标是 了解Vpr在HIV-1免疫发病机制和疾病中的作用 进展申请人建议评价Vpr介导的细胞内增殖的机制。 调节CD4 + T细胞和巨噬细胞反应，并评估 Vpr调节对病毒感染的体内免疫应答的可能性。 具体来说，研究团队将：（1）调查Vpr的直接影响 对体外抗原呈递细胞和T细胞的影响;（2）确定 所述Vpr使用体外免疫模型介导免疫失调; (3)确定Vpr参与介导这些免疫功能的结构域 使用Vpr突变体。拟议的研究将有助于了解如何 Vpr调节这些重要免疫细胞类型中的基本细胞事件， 它如何在体外和体内调节免疫功能。

DESCRIPTION (provided by applicant): The central objective of this project is to understand the role of Vpr in HIV-1 immunopathogenesis and disease progression. The Applicant proposes to evaluate the mechanisms of Vpr-mediated regulation of CD4+ T cell and macrophage responses, and to assess the possibility that Vpr modulates the in vivo immune response to viral infection. Specifically, the research team will: (1) investigate the direct effects of Vpr on antigen presenting cells and T cells in vitro; (2) determine the extent to which Vpr mediates immune dysregulation using an in vitro immunization model; (3) identify the domains of Vpr involved in mediating these immune functions using Vpr mutants. The proposed studies will lead to an understanding of how Vpr modulates basic cellular events in these important immune cell types and how it may regulate immune functions in vitro and in vivo.