Encapsulation of catalytic functionalities into apoferritin for prodrug delivery.

将催化功能封装到脱铁铁蛋白中用于前药递送。

• 批准号: 1923336
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1923336
• 关键词: Encapsulation; catalytic; functionalities; into; apoferritin

Project summary: This project is focused on the design of an artificial enzyme able to cleave ester, carbamate and amide bonds by hydrolysis. These entities will be used to convert prodrug precursors into their pharmacologically active derivatives. Enzymatic hydrolysis plays an important role in prodrug activation. The novelty of this approach is the exploitation of artificial enzymes for the achievement of highly specific targeting in pharmacological therapies in order to improve the efficacy and the toxicological profile of medical treatments. The aim is to use the protein apoferritin, as a biocompatible scaffold for the incorporation of chemical catalysts. Ferritin is an iron storage nanoparticle which has been widely studied for cancer therapeutics and diagnostics. Removal of iron turns ferritin into the apoferritin cage. Ultimately, this project will deliver an engineered apoferritin with the catalytic activity required for prodrugs activation. Objective: design of an artificial enzyme able to catalyse the hydrolysis of compounds containing ester, carbamate and amide groups.Approach: protein engineering and chemical synthesis.Novelty: Use of artificial enzyme as therapeutic tool.

项目概述：本项目的重点是设计一种能够水解酯键、氨基甲酸酯键和酰胺键的人工酶。这些实体将用于将前药前体转化成其生物活性衍生物。酶水解在前药活化中起重要作用。这种方法的新奇在于利用人工酶来实现药物治疗中的高度特异性靶向，以改善医学治疗的功效和毒理学特征。目的是使用脱铁铁蛋白蛋白作为生物相容性支架，用于掺入化学催化剂。铁蛋白是一种铁储存纳米颗粒，已被广泛研究用于癌症治疗和诊断。去除铁使铁蛋白进入脱铁铁蛋白笼。最终，该项目将提供具有前药活化所需的催化活性的工程化脱铁铁蛋白。目的：设计一种能够催化含酯、氨基甲酸酯和酰胺基团的化合物水解的人工酶。2方法：蛋白质工程和化学合成。3新奇：使用人工酶作为治疗工具。