EXTRACELLULAR MATRIX AND AXONAL GUIDANCE IN C. ELEGANS

线虫的细胞外基质和轴突引导

• 批准号: 6539795
• 负责人: WILLIAM G WADSWORTH
• 金额: $ 31.12万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6539795
• 关键词: Caenorhabditis elegans; chimeric proteins; complementary DNA; developmental neurobiology; entactin; extracellular matrix proteins; gene mutation; genetic promoter element; immunocytochemistry; neuroanatomy; neuronal guidance; nucleic acid sequence; site directed mutagenesis

DESCRIPTION (Verbatim From the Applicant's Abstract): Only recently have molecules that guide axon migration been discovered. One of the first was the UNC-6 netrin from the nematode Caenorhabditis elegans. Other guidance molecules interact with and affect UNC-6 activity, and their discovery and the genetic dissection of their interactions are a main focus of this application. Examples include a C. elegans nidogen that acts with UNC-6 to establish the axon scaffold and the identification of a domain within UNC-6 (the C domain) that appears to inhibit axonal branching (branching occurs when this domain is deleted in an UNC-6deltaC construct). The goals of this application are 1) to determine activities associated with other structural modules in UNC-6; 2) to characterize suppressors of UNC-6deltaC; 3) to characterize two other genes that were identified in the screen that identified the C. elegans nidogen; and 4) to identify genes that are required for nidogen to position longitudinal nerves.

描述（来自申请人摘要的逐字记录）：最近才有 引导轴突迁移的分子被发现。第一个是 来自秀丽隐杆线虫的β-6 netrin。其他导向分子 相互作用并影响α-6活性，它们的发现和遗传 对它们的相互作用的剖析是本申请的主要焦点。示例 包括C。与α-6共同作用以建立轴突的线虫巢蛋白 支架和鉴定的结构域内的α-6（C结构域）， 似乎抑制轴突分支（分支发生时，该域是 在β-6deltaC构建体中缺失）。本应用程序的目标是：1） 确定与其他结构模块相关的活动; 2） 表征β_6 δ C的抑制子; 3）表征另外两个基因 在屏幕上识别出了C.线虫巢蛋白;和 4)识别巢蛋白纵向定位所需的基因 神经