EXTRACELLULAR MATRIX AND AXONAL GUIDANCE IN C. ELEGANS

描述(逐字摘自申请人的摘要)：最近才有 引导轴突迁移的分子被发现。第一批人中的一个是 线虫线虫中的UNC-6 netrin。其他导向分子 与UNC-6活性相互作用和影响，以及它们的发现和遗传 分析它们的交互是本应用程序的主要关注点。示例 包括一种与UNC-6作用建立轴突的线虫致病原 脚手架和UNC-6内的域(C域)的标识，该域 似乎抑制轴突分支(当该结构域处于 在UNC-6deltaC构建中删除)。此应用程序的目标是1) 确定与UNC-6中其他结构模块相关的活动；2) 鉴定UNC-6deltaC的抑制子；3)鉴定另外两个基因 在屏幕上识别出线虫致病原的基因；以及 4)确定nidogen纵向定位所需的基因 太紧张了。

DESCRIPTION (Verbatim From the Applicant's Abstract): Only recently have molecules that guide axon migration been discovered. One of the first was the UNC-6 netrin from the nematode Caenorhabditis elegans. Other guidance molecules interact with and affect UNC-6 activity, and their discovery and the genetic dissection of their interactions are a main focus of this application. Examples include a C. elegans nidogen that acts with UNC-6 to establish the axon scaffold and the identification of a domain within UNC-6 (the C domain) that appears to inhibit axonal branching (branching occurs when this domain is deleted in an UNC-6deltaC construct). The goals of this application are 1) to determine activities associated with other structural modules in UNC-6; 2) to characterize suppressors of UNC-6deltaC; 3) to characterize two other genes that were identified in the screen that identified the C. elegans nidogen; and 4) to identify genes that are required for nidogen to position longitudinal nerves.