NEUROCHEMICAL & ANATOMICAL STEPS IN PREOVULATORY GNRH RELEASE

排卵是控制生育的主要目标和 人类和动物的不孕不育。几种神经肽和 神经递质，即神经肽Y、b-内啡肽、去甲肾上腺素 (NE)，γ-氨基丁酸和乙酰胆碱，影响 促性腺激素释放激素的排卵前分泌模式 (GnRH)。基因转录/翻译的大小 表达这些化学物质的神经元可以识别它们如何以及在哪里 调节GnRH的释放。为了检验这一假设，我们利用 早期癌基因CFOS的表达作为定位肿瘤的标志物 脑干和下丘脑基因转录事件的发生 在排卵前促性腺激素释放激素高峰期。雌性兔子的性交，就像 雌激素在猴子体内，在预测的时间诱导NE/GnRH释放 刺激计划。因此，兔子被杀，它们的大脑被修复 多聚甲醛输注前和15、30、60、90和 交配后120min CFOS mRNA定量在S国际电联的应用 杂交。在性交后30分钟内，有几个 去甲肾上腺素能相关脑干核团，外侧被盖(A1)， 孤束核(A2)和蓝斑(A6) 雌性大鼠CFOS基因表达增强。另外，星云中的两个核 延髓向A6核发出投射 舌前部和巨细胞旁核显示 C-fos的表达仅在性交激活的女性中增加。这个 性交后30分钟隔膜也显示出比35S-CFOS更重的标记 来自未交配的对照雌性的隔膜。在下丘脑 前腹侧室周区(AVPV)，弓形，室旁 与内侧视前区相比， 交配后15分钟和60分钟。AVPV显示出最集中的 CFO表达式。这些时间在性交后和转录后 CFO信号的变化可以用于预测地点和 性交刺激如何触发NE/GnRH释放尚不清楚，但这些 数据令人鼓舞。因为下丘脑NE的大量释放 脑干NE(A6)基因的表达增加了两倍 大约在雌激素诱导的下丘脑GnRH释放的时候，我们 提示在兔体内的信号机制可能在 了解灵长类动物体内的这些。资助NIH HD30316、HD18185 出版物无

Ovulation is a major target for the control of fertility and infertility in humans and animals. Several neuropeptides and neurotransmitters, i.e., neuropeptide Y, b-endorphin, norepinephrine (NE), gamma aminobutyric acid and acetylcholine, influence the preovulatory secretion pattern of gonadotropin-releasing hormone (GnRH). The magnitude of gene transcription/translation in the neurons that express these chemicals may identify how and where they modulate GnRH release. To test this hypothesis, we utilized the expression of the early oncogene, cfos, as a marker to locate the onset of gene transcriptional events in brainstem and hypothalamus during the preovulatory GnRH surge. Coitus in female rabbits, like estrogen in monkeys, induces NE/GnRH release at a predicted time after the stimulus. Thus, rabbits were killed and their brains fixed by paraformaldehyde infusion at times before and 15-, 30-, 60-, 90- and 120-min after coitus for cfos mRNA quantitation using in s itu hybridization. Within 30 minutes after coitus, several noradrenergic-associated brainstem nuclei, lateral tegmentum (A1), nucleus of the solitary tract (A2) and locus coeruleus (A6) exhibited enhanced cfos mRNA expression in females. Also, two nuclei in the medulla oblongata that send projections to A6, the nucleus of praepositus hypoglossy and the paragigantocellular nucleus, showed increased c-fos expression only in coitally activated females. The septum also showed heavier 35S-cfos label by 30 min after coitus than septum from unmated control females. In the hypothalamus the anteroventral periventricular area (AVPV), arcuate, paraventricular and medial preoptic area showed enhanced cfos mRNA expression between 15 and 60 minutes postmating. The AVPV showed the most concentrated cfos expression. Whether these times after coitus and transcriptional changes in cfos signals can be utilized for predictions of where and how coital stimulation triggers NE/GnRH release is unclear, but these data are encouraging. Because a massive release of hypothalamic NE and a two-fold increase in brainstem NE (A6) gene expression occurred around the time of the estrogen-induced hypothalamic GnRH release, we suggest that the signaling mechanisms in rabbits may be useful in understanding those in primates. FUNDING NIH HD30316, HD18185 PUBLICATIONS None