ATOMIC RESOLUTION STUDIES OF FERREDOXIN FROM HALOARCULA MARISMORTUI

本实验室的研究主要集中在结构测定上。 (I)细菌通道形成毒素，如葡萄球菌 白血球蛋白(LukF)和(II)配体门控的配体结合域 离子通道。来自F2光束线的数据使我们能够解决 LukF的水溶性结构及其与配体的结合 GluR2的结构域，AMPA选择性谷氨酸受体。两者都有 通过从硒蛋氨酸晶体中进行MAD相变来解决结构问题 分辨率为~2.3。目前，这两个结构都已得到完善 我们目前正在进行稿件的准备工作。从… 这些结构我们在分子水平上加深了我们的理解 水平，细菌毒素经历的构象变化 膜结合和齐聚的结果，我们有 第一次提供了一种细胞外结构域的视图 配基门控离子通道。

The research in my lab has focused on the structure determination of (i) bacterial channel-forming toxins, such as Staphylococcal leukocidin (LukF) and (ii) the ligand binding domain of ligand gated ion channels. Data from the F2 beamline has enabled us to solve the structure of the water-soluble form of LukF and the ligand binding domain of the GluR2, AMPA-selective glutamate receptor. Both structures were solved by MAD phasing from selenomethionine crystals at ~2.3 A resolution. Presently, both structures have been refined and we are currently in the process of manuscript preparation. From these structures we have furthered our understanding, at the molecular level, of the conformational changes that bacterial toxins undergo as a consequence of membrane binding and oligomerization and we have provided, for the first time, a view of the extracellular domain of a ligand gated ion channel.