ATOMIC RESOLUTION STUDIES OF FERREDOXIN FROM HALOARCULA MARISMORTUI

本实验室的研究主要集中在结构测定方面 （i）细菌通道形成毒素，如葡萄球菌毒素， 杀白细胞素（LukF）和（ii）配体门控的配体结合结构域 离子通道 来自F2光束线的数据使我们能够解决 LukF的水溶性形式的结构和配体结合 GluR2，AMPA选择性谷氨酸受体的结构域。 两 通过MAD定相从硒代甲硫氨酸晶体中解析结构 分辨率约为2.3 A。 目前，这两个结构都已完善， 我们目前正在准备手稿。 从 这些结构，我们已经进一步了解，在分子 水平，细菌毒素经历的构象变化， 膜结合和寡聚化的结果， 第一次提供了一个细胞外结构域的视图， 配体门控离子通道

The research in my lab has focused on the structure determination of (i) bacterial channel-forming toxins, such as Staphylococcal leukocidin (LukF) and (ii) the ligand binding domain of ligand gated ion channels. Data from the F2 beamline has enabled us to solve the structure of the water-soluble form of LukF and the ligand binding domain of the GluR2, AMPA-selective glutamate receptor. Both structures were solved by MAD phasing from selenomethionine crystals at ~2.3 A resolution. Presently, both structures have been refined and we are currently in the process of manuscript preparation. From these structures we have furthered our understanding, at the molecular level, of the conformational changes that bacterial toxins undergo as a consequence of membrane binding and oligomerization and we have provided, for the first time, a view of the extracellular domain of a ligand gated ion channel.