Core--Molecular and cellular imaging

核心--分子细胞成像

• 批准号: 6468879
• 负责人: Eric K Richfield
• 金额: $ 13万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6468879
• 关键词: aging; bioimaging /biomedical imaging; biomedical facility; cell type; gene expression; histochemistry /cytochemistry; light microscopy

The Molecular and Cellular Imaging Core (MCIC) will be a local and regional resource for tissue-based image analysis. The MCIC will provide state-of-the-art facilities for qualitative and quantitative film densitometry and microscopic tissue analyses for investigators in the field of aging research. The MCIC will provide training to interested users or perform selected analyses of approved projects. Approved projects are anticipated from local and regional investigators. Applications for MCIC use will additionally solicited form aging-related scientists unbiased stereology, tiled mapping, and 3-D reconstruction. Tissue based techniques including quantitative receptor autoradiography and in situ histochemistry (ISHH) will be performed on a reviewed basis for aging-related projects. These types of analyses and techniques require expertise that may not be available to many laboratories involving in aging studies. Novel in situ hybridization probes and protocols will be developed, validated, and made available to local, regional and national investigators. In collaboration with the Microarray Analysis Core (MAC) and the Gene Expression Vector Core (GEVC) we will develop, validate, and provide five probes to cellular housekeeping genes for ISHH that can be used for validation of data obtained from microarrays. Oligonucleotide or cRNA probes to specific genes of interest to local, regional, or national researchers will be designed or cloned, to be of interest in human tissue or animal models of age-related diseases. These novel genes will be identified by microarray analyses or traditional methods of novel gene identification. As developed, our web site will contain a list of services and probes available to potential users and copies of all protocols for image analyses and ISSH.

分子和细胞成像核心(MCIC)将是一个局部和 基于组织的图像分析的区域资源。MCIC将提供 最先进的定性和定量胶片密度测量设备 为老龄化领域的研究人员提供显微组织分析 研究。MCIC将为感兴趣的用户提供培训或执行 已批准项目的精选分析。预计将批准批准的项目 来自当地和地区调查人员。用于MCIC的应用程序将 另外征求了与衰老相关的科学家不偏不倚的体视学， 平铺映射和三维重建。基于组织的技术包括 定量受体放射自显影和原位组织化学 将对与老龄化有关的项目进行审查。这些 分析和技术的类型需要专业知识，而这些专业知识可能 可供许多参与衰老研究的实验室使用。 将开发新的原位杂交探针和方案， 经过验证，并提供给当地、区域和国家调查人员。 与微阵列分析核心(MAC)和基因 我们将开发、验证和提供表达载体核心(GEVC) ISHH细胞管家基因的五个探针可用于 验证从微阵列获得的数据。寡核苷酸或cRNA 探索地方、地区或国家感兴趣的特定基因 研究人员将被设计或克隆，以使其对人体组织或 年龄相关疾病的动物模型。这些新基因将会是 通过微阵列分析或传统方法鉴定新基因 身份证明。 开发完成后，我们的网站将包含服务和探测器的列表 可供潜在用户使用，以及用于图像分析的所有协议的副本 和ISSH。