CORE--TISSUE CULTURE AND REPOSITORY

皮肤微血管内皮细胞的研究一直受到严重的 由于分离、纯化和系列化的困难， 通过这些细胞。 世界上只有少数实验室 掌握了相关的技术。 此外，人真皮层的培养 微血管内皮细胞是非常昂贵的，因为它们 要求高浓度的人血清。 我们的实验室 微血管内皮细胞培养专家致谢 以及其它更容易获得的皮肤驻留细胞， 角质形成细胞和成纤维细胞。 该核心的具体目标是： 1)提供稳定的新鲜成人和新生儿 人类皮肤给ESDRC调查员。 2)分离、纯化和培养特定群体的常驻细胞 尤其是微血管内皮细胞，以及 来自其他组织的相关细胞，例如大血管内皮细胞和 平滑肌细胞 3)维持和提供永生化微血管内皮细胞 ESDRC调查人员的电话。 4)为ESDRC研究人员提供人体皮肤器官培养， 包括免疫细胞化学和原位杂交。 5)提供其他皮肤常驻细胞的纯培养物， 角质形成细胞、成纤维细胞和平滑肌细胞，如 ESDRC调查员。 6)通过大量采购纸巾提供质量和成本控制 和细胞培养试剂。 7)向ESDRC调查人员提供关于以下技术的培训： 细胞培养和分离以及皮肤器官培养。 8)培养产生抗体的杂交瘤并纯化这些抗体 ESDRC调查员。

The study of cutaneous microvascular endothelial cells has been severely constrained by difficulty in the isolation, purification and serial passage of these cells. Only a handful of laboratories in the world have mastered the techniques involved. Moreover, culture of human dermal microsvacular endothelial cells is extremely expensive, because of their requirements for high concentration of human serum. Our laboratories are acknowledge experts in cell culture of microvascular endothelial cells as well as other more easily obtained resident cells of the skin e.g. keratinocytes and fibroblasts. The specific aims of this core are: 1) To provide a stable supply of freshly harvested adult and neonatal human skin to ESDRC investigators. 2) To isolate, purify and culture specific populations of resident cells of the skin, especially microvascular endothelial cells, as well as relevant cells from other tissues e.g. large vessel endothelial and smooth muscle cells. 3) To maintain and provide immortalized microvascular endothelial cell lines to ESDRC investigators. 4) To provide organ culture of human skin to ESDRC investigators for studies involving immunocytochemistry and in situ hybridization. 5) To provide pure cultures of other resident cells of skin such as keratinocytes, fibroblasts, and smooth muscle cells, as requested by ESDRC investigators. 6) To provide quality and cost control through bulk purchases of tissue and cell culture reagents. 7) To provide training to ESDRC investigators regarding techniques of cell culture, and isolation as well as skin organ culture. 8) To culture antibody producing hybridomas and purify these antibodies for ESDRC investigators.