CORE--METABOLISM LABORATORIES

核心单元的目标是提供持续的高质量 为PERC的代谢研究提供分析支持。这一目标可以 以经济高效的方式实现，通过拥有 中央核心设施由多名调查人员使用。这 对于稳定的同位素和质谱学是特别必要的， 由于方法的复杂性和高昂的成本 采购和维护必要的设备。在过去的时间里 几年来，所有这些目标都成功实现了。 核心新陈代谢实验室对 不同研究人员的工作效率和研究质量 PERC。GC-MS实验室已经开发出新的创新稳定同位素 妊娠受试者代谢的研究方法和方法 新生婴儿。下列稳定同位素的分析技术 我们的实验室建立了标记底物：葡萄糖、尿素、 丙氨酸、亮氨酸、赖氨酸、丝氨酸和甘氨酸、乳酸、棕榈酸、 甘油和酮。所有的方法现在都是常规分析。在……里面 此外，测量~(13)C丰度的分析技术 血液中的碳酸氢盐已经被提炼了。 最近增加的一项是α-酮酸的定量分析。 支链氨基酸的GC-MS和高效液相色谱图谱。这些技术是 重要的是为了测量前驱体库的浓缩 量化蛋白质合成。 提供的服务将包括样本分析、口译帮助、 监督咨询。核心还负责 维护和充分发挥精密分析系统的功能 乐器。所有使用稳定同位素的新陈代谢方案 示踪剂将使用核心实验室。

The objective of the Core unit is to provide continued high quality analytical support for the metabolic studies in the PERC. This aim can be achieved efficiently and in a cost-effective way, by having a centralized Core facility utilized by a number of investigators. This is particularly necessary for stable isotope and mass spectrometry, because of the complicated nature of methods and the high cost of purchasing and maintaining the necessary equipment. Over the past several years, all of these objectives have been successfully achieved. The Core Metabolism Laboratories have had a major impact on the productivity and the quality of research of various investigators in the PERC. The GC-MS Laboratory has developed new innovative stable isotope methods for the study of metabolism in the pregnant subject and the newborn infants. Analytical techniques for the following stable isotope labelled substrates are established in our laboratories: glucose, urea, alanine, leucine, lysine, serine and glycine, lactate, palmitic acid, glycerol, and ketones. All of the methods are now routine analysis. In addition, the analytical technique for the measurement of 13C enrichment of blood bicarbonate has been refined. A more recent addition has been the quantitative analysis of a-keto acids of branched-chain amino acids on GC-MS & HPLC. These techniques were important in order to measure the precursor pool enrichment for quantifying protein synthesis. Services provided will include sample analysis, help in interpretation, supervision and consultation. The Core is also responsible for the maintenance and adequate functioning of the sophisticated analytical instruments. All of the metabolism protocols utilizing stable isotope tracers will use the Core Laboratory.