Schizosaccharomyces pombe Ribonuclease P

描述(申请人提供)：核糖核酸酶P(RNaseP)是一种 核糖核蛋白颗粒(RNP)，在转录前核糖核酸的加工过程中起作用。 虽然RNaseP是所有细胞中必不可少的并且存在于所有细胞中，但这个RNP的结构 显示出相当大的多样性。细菌全酶由一个大的 RNA分子和一种小的多肽。细菌核糖核酸酶P-RNA已被证明 要成为核酶，也就是说，在没有蛋白质的情况下，RNA是催化的。在……里面 相比之下，发芽酵母的全酶是 显示由一个RNA分子和至少九个多肽组成。的活动 在没有蛋白质的情况下，真核生物的核糖核酸酶P RNA从未被 演示了。另一种真核糖核酸酶P的组成，即裂变的组成 据预测，酵母菌与裂殖酵母有很大不同， 由一个RNA分子和一个100kD的多肽组成。然而， 几种酿酒酵母核糖核酸酶P蛋白的同源物已被鉴定 在S的庞贝基因组中表明这些酶可能比 最初认为。这一提议需要更完整的生化反应 金黄色葡萄球菌核糖核酸酶P的纯化及性质研究 金黄色葡萄球菌核糖核酸酶P蛋白的生物化学研究 重组实验和基因缺失分析。提纯和 对S.pombe核糖核酸酶P的鉴定将为进一步的研究奠定基础 真核核糖核酸酶的复杂性研究。

DESCRIPTION (provided by applicant): Ribonuclease P (RNase P) is a ribonucleoprotein particle (RNP) that functions in the processing of pre-tRNAs. While RNase P is essential and present in all cells, the structure of this RNP exhibits considerable diversity. The bacterial holoenzyme consists of a large RNA molecule and a small polypeptide. The bacterial RNase P RNA has been shown to be a ribozyme, that is, the RNA is catalytic in the absence of protein. In contrast, the holoenzyme from the budding yeast Saccharomyces cerevisiae was shown to consist of an RNA molecule and at least nine polypeptides. Activity of RNase P RNA from eucaryotes in the absence of protein has never been demonstrated. The composition of another eucaryal RNase P, that of the fission yeast Schizosaccharomyces pombe, has been predicted to be very different, consisting of an RNA molecule and a single 100 kD polypeptide. However, homologs of several of the S. cerevisiae RNase P proteins have been identified in the S pombe genome suggesting these enzymes may be more similar than initially believed. This proposal entails a more complete biochemical purification and characterization of the S. pombe RNase P. The function of the S. pombe RNase P proteins will be investigated by both biochemical reconstitution experiments and genetic deletion analysis. The purification and characterization of the S. pombe RNase P will provide groundwork for further study of the complexity of eucaryal RNase P.