Vaccines Designed From Analysis of Recent HIV Infections

根据近期艾滋病毒感染分析设计的疫苗

• 批准号: 6550706
• 负责人: Phillip Wayne Berman
• 金额: $ 21万
• 依托单位: VAXGEN, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6550706
• 关键词: AIDS vaccines; HIV envelope protein gp120; HIV infections; clinical research; computer assisted sequence analysis; computer program /software; computer system design /evaluation; disease /disorder onset; guinea pigs; human genetic material tag; human tissue; molecular biology information system; neutralizing antibody; polymerase chain reaction; protein sequence; virus genetics

DESCRIPTION (provided by applicant): An effective vaccine is the only practical way to halt the AIDS epidemic. The greatest challenge in developing an effective HIV vaccine is antigenic variation. Therefore, the present project aim is to enhance the understanding of antigenic variation by characterizing intra-subtype sequence variation among gpl20 genes from incident (new) infections, and identifying novel antigens that will be manufactured and advanced into clinical trails. Samples will be collected from VaxGen's current Phase III U.S. AIDS vaccine clinical trials. Amino acid sequences will be analyzed using new software that maps sequence variation onto the 3D crystal structure of gp120 to define substitutions that may alter the antigenic profile. This study will provide a database of virus sequences currently circulating throughout North America, with reference to approximate time of infection, geographic distribution, and the nature of source material. These data will aid in incorporating new antigens to improve current HIV vaccine efficacy against variants of subtype B by guiding selection of the most appropriate envelope antigens for next generation HIV vaccines.

描述（由申请人提供）：有效的疫苗是制止艾滋病流行的唯一实际途径。研制有效的艾滋病毒疫苗的最大挑战是抗原变异。因此，本项目的目的是通过表征突发（新）感染的gpl20基因的亚型内序列变异，并识别将被制造并进入临床试验的新抗原，从而增强对抗原变异的理解。样本将从VaxGen目前的美国艾滋病疫苗III期临床试验中收集。氨基酸序列将使用新的软件进行分析，该软件将序列变化映射到gp120的3D晶体结构上，以确定可能改变抗原谱的替换。这项研究将提供目前在北美流行的病毒序列数据库，并参考感染的大致时间、地理分布和源材料的性质。这些数据将通过指导下一代艾滋病毒疫苗选择最合适的包膜抗原，帮助纳入新的抗原，以提高目前针对B亚型变体的艾滋病毒疫苗的效力。