The BAM in bugs - understanding bacteria using a chemical biology toolbox. (SEARCEY_U17DTP)

细菌中的 BAM - 使用化学生物学工具箱了解细菌。

• 批准号: 1937452
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1937452
• 关键词: BAM; bugs; understanding; bacteria; using

Antimicrobial resistance remains a frightening prospect. It has been suggested that, should we fail to find new ways to kill bacteria, we will return to the days when a routine operation could prove fatal. We need to understand how bacteria function. This research project is an exciting approach to the study of the fundamental biology of Gram negative bacteria using a combination of synthetic chemistry, structural biology and microbiology. These bacteria have to display proteins (called outer membrane proteins) on their surface and to do this they use a molecular machine known as the BAM complex. Research from Professor Chiangjang Dong at UEA has led the world in understanding the BAM complex through the use of crystallography. In collaboration with Professor Mark Searcey, we will now design and synthesise peptides and small molecules that will allow us to manipulate this complex and to understand how it functions and how it could potentially become a target for the design of new antimicrobial agents. The project will require skills in synthetic organic chemistry, peptide and small molecule synthesis and an interest in developing skills in crystallography and microbiology.

抗生素耐药性仍然是一个可怕的前景。有人认为，如果我们找不到杀死细菌的新方法，我们将回到常规手术可能致命的日子。我们需要了解细菌的功能。这个研究项目是一个令人兴奋的方法，使用合成化学，结构生物学和微生物学的组合来研究革兰氏阴性菌的基础生物学。这些细菌必须在其表面展示蛋白质（称为外膜蛋白），为此它们使用称为BAM复合物的分子机器。东英吉利大学的Chiangjang Dong教授的研究通过使用晶体学引领世界了解BAM复合体。在与Mark Searcey教授的合作中，我们现在将设计和合成肽和小分子，使我们能够操纵这种复合物，并了解它的功能以及它如何可能成为新抗菌剂设计的目标。该项目将需要在合成有机化学，肽和小分子合成的技能，并在晶体学和微生物学发展技能的兴趣。