Pathophysiology of Chronic Cerebral Vasospasm

慢性脑血管痉挛的病理生理学

• 批准号: 6547305
• 负责人: Robert Loughlin Macdonald
• 金额: $ 59.88万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6547305
• 关键词: Macaca fascicularis; cGMP dependent protein kinase; calcium flux; cerebral aneurysm; cerebral artery; cerebral hemorrhage; clinical research; disease /disorder model; electron spin resonance spectroscopy; hemoglobin; immunocytochemistry; laboratory rat; nitric oxide; pathologic process; polymerase chain reaction; potassium channel; thrombosis; tissue /cell culture; vascular endothelium; vascular smooth muscle; vasodilation; vasospasm; voltage /patch clamp; western blottings

DESCRIPTION (provided by applicant): We are investigating cerebral vasospasm which is an important cause of cerebral ischemia after subarachnoid hemorrhage (SAH). The long-term objective of this grant is to determine the mechanism of vasospasm after SAH and to thereby develop treatments that will prevent and/or reverse it. We have shown that hemoglobin causes vasospasm and that vasospasm is associated with impaired arterial relaxation. One mechanism of hemoglobin-induced vasospasm may be the binding and removal of nitric oxide (NO). We have used electron paramagnetic resonance (EPR) spectroscopy to detect nitrosyl hemoglobin in the subarachnoid space after SAH, proving that this mechanism occurs. We will therefore test the hypothesis that there is an NO-reversible component of vasospasm by: 1) defining the extent to which vasospasm is reversible with NO donors in a monkey model of SAH; 2) measuring heme-NO adducts (nitrosyl hemoglobin) by EPR spectroscopy in clots removed from the subarachnoid space of monkeys at different times after SAH; 3) quantifying NO in the perivascular space at different times after SAH in monkeys; and 4) defining the time course of changes in and the immunohistochemical locations of the 3 isoforms of NOS in cerebral arteries and perivascular blood clot after SAH in monkeys. Second, because vasospasm does not seem to be completely preventable by NO donors, we will investigate mechanisms of NO-independent vasospasm by: 1) measuring protein kinase G messenger ribonucleic acid, protein and activity during the time course of vasospasm in monkeys; and 2) assessing calcium sensitivity of monkey cerebral arteries during the time course of vasospasm. In a rat model, we will assess the contribution of other downstream effectors of NO-induced relaxation by: 1) assessing potassium channel function during vasospasm (calcium-activated potassium channel density, single channel conductance, and open probability will be assessed using whole cell and single channel patch clamp recordings of isolated vasospastic rat cerebrovascular smooth muscle cells); and 2) measuring whole cell calcium currents during vasospasm in rats because assessment of potassium channel function requires knowledge of intracellular calcium and because smooth muscle calcium homeostasis may be altered during vasospasm after SAH.

描述（由申请人提供）： 我们正在研究脑血管痉挛，这是一个重要的原因，脑缺血后蛛网膜下腔出血（SAH）。该基金的长期目标是确定蛛网膜下腔出血后血管痉挛的机制，从而开发预防和/或逆转血管痉挛的治疗方法。我们已经证明血红蛋白可引起血管痉挛，血管痉挛与动脉舒张功能受损有关。血红蛋白引起的血管痉挛的机制之一可能是一氧化氮（NO）的结合和清除。我们已经使用电子顺磁共振（EPR）光谱检测蛛网膜下腔后的亚硝基血红蛋白，证明这种机制的发生。因此，我们将通过以下方法检验血管痉挛中存在NO可逆成分的假设：1）在猴SAH模型中确定NO供体可逆转血管痉挛的程度; 2）测量血红素-NO加合物在SAH后不同时间从猴蛛网膜下腔取出的凝块中通过EPR光谱测定（亚硝基血红蛋白）;（3）测定猴SAH后不同时间血管周围NO含量，（4）确定猴SAH后脑动脉和血管周围血凝块中NOS三种亚型的变化时间和免疫组织化学定位。其次，由于血管痉挛似乎不能完全由NO供体预防，我们将通过以下方式研究NO非依赖性血管痉挛的机制：1）测量猴血管痉挛时程中的蛋白激酶G信使核糖核酸、蛋白质和活性;和2）评估血管痉挛时程中猴脑动脉的钙敏感性。在大鼠模型中，我们将通过以下方式评估NO诱导舒张的其他下游效应物的作用：1）评估血管痉挛期间钾通道功能（将使用分离的血管痉挛大鼠脑血管平滑肌细胞的全细胞和单通道膜片钳记录来评估钙激活钾通道密度、单通道电导和开放概率）;和2）测量大鼠血管痉挛期间的全细胞钙电流，因为钾通道功能的评估需要了解细胞内钙，并且因为在SAH后血管痉挛期间平滑肌钙稳态可能改变。