Core--Morphology

Morphology Core已经进行了广泛的重组，以响应 原申请审查员的关注。的总体目标 该设施仍然能够实现空间关系的可视化， 细胞和亚细胞成分的胃肠道，通过提供技术 服务，咨询，并在光形态方法的培训 和电子显微镜水平的分辨率。一个主要目标是提供 所有DDRCC成员都有在一个 及时和低成本。该设施由以下专家指导： 鲁宾博士（主任）和洛伦兹（联合主任），他们都有广泛的 在GI形态学和各种专业形态学方面的经验 技术.这代表了对原始应用程序的更改，其中 Kevin Roth博士（现任分子生物学和药理学主任） Morphology Core）是该设施的唯一董事。资源包括 三个组成部分：组织学、共聚焦显微镜和电子显微镜。它 预计组织学将是订阅量最大的组成部分， 核心，预计有27个用户。组织学服务（石蜡包埋 切片、特殊染色、冷冻切片的制备） 位于胃肠病学分部的一个新站点，紧邻 鲁宾博士的实验室和许多DDRCC成员的实验室附近。这 改变被认为是确保质量控制的最佳方式。 的服务.这个网站目前是组织学和光装备精良 显微镜，因此不需要大量的启动预算。因为 共聚焦显微镜和电子显微镜的当前和预计使用是 相对较低（预计分别为9个和12个用户），单独的网站 将不会为这些服务提供支持。已作好安排 使DDRCC成员能够使用共聚焦成像设施和电子 显微镜设施（由罗伯特威尔金森博士和玛丽莲利维管理， 分别）在细胞生物学和生理学系在减少 费用（由复员方案协调委员会资金补贴）。John Heuser博士，细胞生物学教授 和生理学和成像技术的领先专家，以及凯文罗斯博士 他是分子生物学和药理学形态学中心的主任， 将担任复员方案协调中心的顾问。

The Morphology Core has been extensively restructured in response to the concerns of the reviewers of the original application. The overall goal of the facility remains to enable visualization of the spatial relationships between both cellular and subcellular components of the GI tract by providing technical services, consultation, and training in morphological approaches at the light and electron microscope levels of resolution. A major objective is to provide all DDRCC members with services for embedding and sectioning of GI tissues in a timely manner and at low cost. The facility is under the expert direction of Drs. Rubin (director) and Lorenz (co-director), both of whom have extensive experience in GI morphology and in a variety of specialized morphological techniques. This represents a change from the original application, in which Dr. Kevin Roth (current director of the Molecular Biology and Pharmacology Morphology Core) was sole director of this Facility. The resource consists of three components: histology, confocal microscopy, and electron microscopy. It is anticipated that histology will be the most heavily subscribed component of the Core, with 27 projected users. Histological services (paraffin-embedding and sectioning, special stains, preparation of cryosections) will be provided at a new site located in the Division of Gastroenterology, immediately adjacent to Dr. Rubin's laboratory and near the laboratories of many DDRCC members. This change is believed to be the best way to ensure quality control over the service. This site is currently well-equipped for histology and light microscopy, so an extensive start-up budget will not be required. Because the current and projected use of confocal microscopy and electron microscopy is relatively low (nine and 12 projected users, respectively), a separate site will not be maintained for these services. Arrangements have been made to enable DDRCC members to use the Confocal Imaging facility and Electron Microscopy facility (administered by Dr. Robert Wilkinson and Marilyn Levy, respectively) in the Department of Cell Biology and Physiology at a reduced cost (subsidized by DDRCC funds). Dr. John Heuser, Professor of Cell Biology and Physiology and a leading expert in imaging techniques, and Dr. Kevin Roth who is the director of the Molecular Biology and Pharmacology Morphology Core will serve as consultants for the DDRCC Facility.