Core--Morphology

形态核心进行了广泛的重组，以应对 原始申请的审查者的关切。该计划的总体目标 设施保留，以实现可视化之间的空间关系 胃肠道的细胞和亚细胞成分，通过提供技术 在光的形态方法方面的服务、咨询和培训 和电子显微镜的分辨率水平。一个主要目标是提供 所有具有GI组织植入和切片服务的DDRCC成员 及时，成本低。该设施在专家的指导下 鲁宾博士(导演)和洛伦茨博士(联合导演)，他们两人都有广泛的 有胃肠道形态学和各种专业形态学方面的经验 技巧。这表示与原始应用程序不同，其中 凯文·罗斯博士(现任分子生物学和药理学主任 形态核心)是该机构的唯一主管。该资源包括 三个组成部分：组织学、共聚焦显微镜和电子显微镜。它 预计组织学将是订阅最多的组件 核心，有27个预计用户。组织学服务(石蜡包埋 并提供切片、特殊染色、冰冻切片的准备) 在位于消化内科的一个新地点，紧挨着 鲁宾博士的实验室和许多DDRCC成员的实验室附近。这 改变被认为是确保对 服务。该网站目前在组织学和光线方面装备良好。 显微镜，因此不需要大量的启动预算。因为 目前和预计共聚焦显微镜和电子显微镜的使用情况是 相对较低(分别为9和12个预计用户)，一个单独的站点 不会为这些服务维护。已作出安排，以 使DDRCC成员能够使用共焦成像设备和电子设备 显微镜设备(由Robert Wilkinson博士和Marilyn Levy管理， 分别)在细胞生物学和生理学系 费用(由DDRCC基金补贴)。约翰·豪泽博士，细胞生物学教授 生理学和成像技术方面的领先专家，以及凯文·罗斯博士 谁是分子生物学和药理学形态核心的主任 将担任解除武装、复员和重返社会中心机制的顾问。

The Morphology Core has been extensively restructured in response to the concerns of the reviewers of the original application. The overall goal of the facility remains to enable visualization of the spatial relationships between both cellular and subcellular components of the GI tract by providing technical services, consultation, and training in morphological approaches at the light and electron microscope levels of resolution. A major objective is to provide all DDRCC members with services for embedding and sectioning of GI tissues in a timely manner and at low cost. The facility is under the expert direction of Drs. Rubin (director) and Lorenz (co-director), both of whom have extensive experience in GI morphology and in a variety of specialized morphological techniques. This represents a change from the original application, in which Dr. Kevin Roth (current director of the Molecular Biology and Pharmacology Morphology Core) was sole director of this Facility. The resource consists of three components: histology, confocal microscopy, and electron microscopy. It is anticipated that histology will be the most heavily subscribed component of the Core, with 27 projected users. Histological services (paraffin-embedding and sectioning, special stains, preparation of cryosections) will be provided at a new site located in the Division of Gastroenterology, immediately adjacent to Dr. Rubin's laboratory and near the laboratories of many DDRCC members. This change is believed to be the best way to ensure quality control over the service. This site is currently well-equipped for histology and light microscopy, so an extensive start-up budget will not be required. Because the current and projected use of confocal microscopy and electron microscopy is relatively low (nine and 12 projected users, respectively), a separate site will not be maintained for these services. Arrangements have been made to enable DDRCC members to use the Confocal Imaging facility and Electron Microscopy facility (administered by Dr. Robert Wilkinson and Marilyn Levy, respectively) in the Department of Cell Biology and Physiology at a reduced cost (subsidized by DDRCC funds). Dr. John Heuser, Professor of Cell Biology and Physiology and a leading expert in imaging techniques, and Dr. Kevin Roth who is the director of the Molecular Biology and Pharmacology Morphology Core will serve as consultants for the DDRCC Facility.