CORE--AUTOMATED DNA SEQUENCING FACILITY

最近在麦卡德尔建立的自动DNA测序设备 癌症研究实验室为科学家提供了访问最新技术的途径 用于快速DNA测序的ART机器。这项计划的所有五个项目- 项目赠款目前大量使用这一共同设施。这个 预计未来几年对这一设施的需求将会增加。用途 包括重组DNA序列验证、候选DNA的筛选 来自大型项目的资金池，如酵母双杂交和SELEX 肿瘤抑制基因和癌基因的程序和序列分析 在实验动物肿瘤中。需要资金来支持这笔费用 运营这个设施与项目使用的比例成比例 由这项计划-项目拨款支持。仅限于运营成本 常见机器上的样本和这些机器的维护 根据这一核心提出的要求。所有其他准备费用 对样本进行排序是由单个项目引起的。

The recently established Automated DNA Sequencing Facility in the McArdle Laboratory for Cancer Research provides scientists access to state-of-the- art machines for rapid DNA sequencing. All five projects of this program- project grant currently make heavy use of this common facility. The projected needs for this facility will increase in future years. Uses include sequence verification of recombinant DNAs, screening of candidate pools from large-scale projects such as yeast two-hybrid and SELEX procedures, and sequence analysis of tumor suppressor genes and oncogenes in experimental animal tumors. Funds are requested to support the costs for running this facility proportional to its use by the projects supported by this program-project grant. Only costs incurred for running samples on the common machines and the maintenance of those machines are requested under this core. All other costs for the preparation of sequencing samples are incurred by individual projects.