Core--Molecular and bioanalytical facility

核心--分子和生物分析设施

• 批准号: 6643659
• 负责人: MARIANNE GARLAND
• 金额: $ 18.37万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6643659
• 关键词: biomedical facility; enzyme linked immunosorbent assay; growth /development; high performance liquid chromatography; polymerase chain reaction

The Molecular and Bioanalytical Core is expected to be used extensively by each of the four research projects for the assays of numerous substances and RNA/DNA typing by PCR. Based on the organization and description of this Core provided in the application, it appears that its objective, to provide resources, expertise, and technical support in an efficient and cost effective manner, would be achieved in this program. Core B is a new addition to the Program and is expected to enhance the productivity of the combined research projects, encourage new enterprises among the projects, and be a more cost effective approach to achieve the objectives of the individual projects. Based on the description of the assays provided in the application, the quality of the services appears to be good; however, quality control procedures are not specifically addressed in the application. The oversight of the actual analyses in the Core laboratory would be divided between three individuals: Dr. Daniel, who has provided the mainstay of basic biochemical and analytic assays over the course of the Program, would continue in this capacity to assure quality control and perform general biochemistry and radioimmunoassays, and this is a strength of the proposed Core; Dr. Vosatka, who has considerable experience with molecular biology, would oversee the molecular biology analyses as well as the cell and tissue culture work; and the overall direction of Core B would be by Dr. Garland, who also would oversee the analytical pharmacology (e.g. HPLC, ELISA) and steroid and hormonal assays. It is not evident from Dr. Garland s biographical sketch that she has had particular experience in this area.

分子和生物分析核心预计将广泛用于 四个研究项目中的每一个都用于分析多种物质， 通过PCR进行RNA/DNA分型。根据本核心的组织和描述， 在应用程序中提供，它似乎是其目的，以提供 资源、专业知识和技术支持， 这一点，将在该计划中实现。核心B是 计划，并预计将提高生产力的结合研究 项目，鼓励新的企业之间的项目，并将更多的成本 有效的方法来实现个别项目的目标。 基于本申请中提供的测定的描述， 服务质量似乎很好;但是，质量控制 申请中没有具体说明程序。 对核心实验室实际分析的监督将分为 三个人之间：丹尼尔博士，谁提供了基本的支柱， 生物化学和分析化验在该计划的过程中，将继续 以确保质量控制和进行一般生物化学 和放射免疫测定，这是一个拟议的核心优势;博士。 沃萨特卡在分子生物学方面有着丰富的经验， 分子生物学分析以及细胞和组织培养工作; 核心B的总体方向将由加兰博士负责，他还将 监督分析药理学（例如HPLC，ELISA）和类固醇， 激素测定。从加兰博士的传记中看不出 她在这方面有特别的经验