IMMUNE PEPTIDES IN AEDES AEGYPTI

描述（改编自申请人的摘要）：该提案来自 一个新的研究人员是针对4的表征 免疫激活的Ae.埃及蚊子。使用MALDI-TOF MS与P. Bulet（法国国家科学研究中心，斯特拉斯堡）合作，PI 获得了至少15种免疫诱导肽的证据。 埃及人。初步数据支持分析天蚕素（CEC）， 对革兰氏阴性菌（GR）有活性的蛋白质，富含脯氨酸的 肽（PRO）和690 Da分子（M690）。编码这四种基因的基因 分子将被克隆，使用PCR和相关技术， 将测定肽的抗微生物谱。最后 蛋白质将使用辛德毕斯载体在蚊子中表达， 蚊子随后会接触到传染性的血液 （鸡疟原虫和马来丝虫）以确定 单独和组合的肽对寄生虫发育的影响。

DESCRIPTION (Adapted from the Applicant's Abstract): This proposal from a new investigator is directed towards the characterization of 4 molecules from immune-activated Ae. aegypti mosquitoes. Using MALDI-TOF MS in collaboration with P. Bulet (CNRS, Strasbourg, France), the PI has obtained evidence for at least 15 immune-inducible peptides in Ae. aegypti. Preliminary data support the analysis of a cecropin (CEC), a protein active against Gram negative bacteria (GR), a proline-rich peptide (PRO) and a 690 Da molecule (M690). Genes encoding these four molecules will be cloned, using PCR and related technologies, and the antimicrobial spectrum of the peptides will be determined. Finally, the proteins will be expressed in mosquitoes using a Sindbis vector, and the mosquitoes will subsequently be exposed to an infectious bloodmeal (Plasmodium gallinaceum and Brugia malayi) to determine the effects of the peptides, alone and in combination, on parasite development.