REGULATION OF FLAGELLUM AND PFT IN LEISHMANIA

利什曼原虫鞭毛和 PFT 的调控

• 批准号: 6534280
• 负责人: JAMES D FORNEY
• 金额: $ 23.5万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6534280
• 关键词: Leishmania; cell component structure /function; cell motility; flagellum; life cycle; messenger RNA; microorganism culture; microorganism growth; protein transport

The overall issue addressed by this proposal is to understand regulatory processes governing stage-specific elaboration of the flagellum in Leishmania mexicana. A profound change in flagellar morphology is one of the features distinguishing the insect and mammalian stages of the human pathogen Leishmania. Despite classical recognition of these changes in flagellar morphology occurring curing the life cycle, very little is known about how the structural components of the flagellum are assembled and disassembled in correspondence with the life cycle. Focusing on the paraflagellar rod, this proposal describes experiments to study the regulatory mechanisms deployed by these parasites to control production and demolition of the flagellum and to understand the role played by this motility organelle in the biology of the organism. We will explore regulation at the level of mRNA production, translation, protein transport to the parasites' biology could reveal new targets for antiparasitic therapies. The functional importance of the PFR coupled with its proven potential as a vaccine candidate justify a more complete characterization of this complex structure.

这个建议所解决的总体问题是要了解墨西哥利什曼原虫鞭毛的特定阶段的制定管理过程。 鞭毛形态的深刻变化是区分人类病原体利什曼原虫的昆虫和哺乳动物阶段的特征之一。 尽管这些鞭毛形态的变化发生固化的生活周期的经典认识，很少有人知道鞭毛的结构组件是如何组装和拆卸对应的生活周期。 专注于paraflagellar杆，该提案描述了实验来研究这些寄生虫部署的监管机制，以控制生产和拆除的鞭毛，并了解这个能动性细胞器在生物体中所发挥的作用。 我们将探索在mRNA产生、翻译、蛋白质转运水平上对寄生虫生物学的调控，可能揭示抗寄生虫治疗的新靶点。 PFR的功能重要性加上其作为疫苗候选物的经证实的潜力证明了对这种复杂结构的更完整表征是合理的。