ANESTHETIC ACTION ON NEUROTRANSMITTER RESPONSES
对神经递质反应的麻醉作用
基本信息
- 批准号:6501515
- 负责人:
- 金额:$ 8.62万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-08-01 至 2002-07-31
- 项目状态:已结题
- 来源:
- 关键词:GABA receptor acetylcholine anesthesia anesthetics cell line chemical binding chemical structure clone cells drug interactions electrophysiology fibroblasts inhibitor /antagonist membrane channels molecular site neural transmission neurons neurotransmitters receptor expression tissue /cell culture tissue mosaicism voltage /patch clamp
项目摘要
This project will examine the actions of steroid anesthetics on
transmitter-gatedmembrane channels. The experiments are designed
to determine the sites on receptors to which the steroids bind, and
define the mechanism(s) by which functional effects are mediated.
The main focus of the proposed work is the gamma-amino butyric acid
type-A receptor (GABA-A receptor), which has been proposed as a
major site of action for general anesthetics, including steroids. Defined
combinations of subunits will be expressed in non-neural cells.
Molecular biological techniques will be used to manipulate the
structure of the expressed subunits, to define regions of GABA-A
receptor subunits required for anesthetic action. Complementary work
will use pharmacological and biophysical approachers to examine the
mechanism for anesthetic action. Stable clonal cell lines expressing
high levels of GABA-A receptors will be generated for use in
physiological and biochemical studies. The studies of GABA-A
receptors will be extended by comparative data obtained for anesthetic
actions on the major brain nicotinic receptor, stably expressed in non-
neural cells. Finally, the actions of the intravenous anesthetics
pentobarbital and propofol will be compared to those of steroids in
these experiments. The results should lead to insights into the
mechanisms by which steroid anesthetics act at one molecular target,
the GABA-A receptors. Comparisons with other data obtained in this
project and the Program as a whole will indicate the range of effects
which anesthetics have on several identified membrane channels
involved in synaptic transmission. This information is required for a
better understanding of the cellular actions associated with anesthesia.
本项目将研究类固醇麻醉药的作用
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Joseph H. Steinbach其他文献
High blood cholesterol reduces in vitro blood oxygen delivery.
高血液胆固醇会减少体外血液氧输送。
- DOI:
- 发表时间:
1974 - 期刊:
- 影响因子:2.2
- 作者:
Joseph H. Steinbach;P. L. Blackshear;R. L. Varco;Henry Buchwald - 通讯作者:
Henry Buchwald
Measurement of gastric bicarbonate secretion in the human stomach: different methods produce discordant results.
人胃中胃碳酸氢盐分泌的测量:不同的方法产生不一致的结果。
- DOI:
10.3109/00365529209000149 - 发表时间:
1992 - 期刊:
- 影响因子:1.9
- 作者:
H. Odes;D. Hogan;Joseph H. Steinbach;M. Ballesteros;M. Koss;Jon I. Isenberg - 通讯作者:
Jon I. Isenberg
Joseph H. Steinbach的其他文献
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{{ truncateString('Joseph H. Steinbach', 18)}}的其他基金
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