Generating distinct actin networks in a single cytoplasm: Structural studies to determine the impact of actin nucleators on filament structure

在单个细胞质中生成不同的肌动蛋白网络：确定肌动蛋白成核剂对丝结构影响的结构研究

• 批准号: 1944851
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1944851
• 关键词: Generating; distinct; actin; networks; single

A fundamental understanding of how a cell responds to its environment, in order to drive changes to cell physiology, is critical if we are to make relevant and appropriate interventions in the context of disease states. Many years of research have demonstrated that the actin cytoskeleton is a focal point of cell regulation, however there are still large gaps in our understanding of mechanisms governing early steps in actin filament formation and how distinct filament organizations can co-exist in a single cytoplasm. This project will combine molecular biology and biochemistry with advanced electron cryomicroscopy (CryoEM) approaches to gain new structural insights into the process of actin nucleation. It will be supported by world-class labs with expertise in actin biochemistry and cell biology (Ayscough) and in structural analysis of macromolecular complexes using CryoEM (Bullough).

如果我们要在疾病状态的背景下进行相关和适当的干预，对细胞如何对其环境做出反应的基本了解，以推动细胞生理学的变化，是至关重要的。多年来的研究表明，肌动蛋白细胞骨架是细胞调控的焦点，然而，对于肌动蛋白微丝形成的早期机制以及不同的微丝组织如何共存于单个细胞质中，我们的理解仍然存在很大差距。这个项目将结合分子生物学和生物化学与先进的电子冷冻显微镜(CryoEM)方法，以获得对肌动蛋白成核过程的新的结构见解。它将得到在肌动蛋白生物化学和细胞生物学(Ayscough)以及使用低温EM(Bullough)进行大分子复合体结构分析方面专业知识的世界级实验室的支持。