Core--Biological assessment laboratory

核心--生物评估实验室

• 批准号: 6501871
• 负责人: Mary A Fletcher
• 金额: $ 20.68万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6501871
• 关键词: analytical chemistry; biomedical facility; human tissue; psychological aspect of cancer; psychoneuroimmunology

The Laboratory Core will provide the assessments of soluble mediators, lymphocyte surface markers, hematological parameters and lymphocyte function in support of the scientific agenda of this Center. The following assays will be done by the Laboratory Core: Cortisol and catecholamines, norepinephrine (NE) and epinephrine (E) in urine. Testosterone, estradiol,. Estrone, sex hormone binding globulin (SHBG) and dehydroepiandrosterone-sulfate (DHEA-S) and NPY in plasma. Natural killer cell cytotoxicity (NKCC) against the tumor cell target, K562. NKCC against the tumor cell target, K562, after ex vivo exposure to interferon-gamma (IFN-gamma). Number and percent of NK (C56+CD3-), CD3+CD4+ and CD3+CD8+ lymphocytes using 4 color flow cytometry.

实验室核心将提供可溶性介质、淋巴细胞表面标志物、血液学参数和淋巴细胞功能的评估，以支持该中心的科学议程。将由实验室核心进行以下测定：尿中的皮质醇和儿茶酚胺、去甲肾上腺素（NE）和肾上腺素（E）。睾酮雌二醇。血浆雌酮、性激素结合球蛋白（SHBG）、硫酸脱氢表雄酮（DHEA-S）和神经肽Y。针对肿瘤细胞靶点K562的自然杀伤细胞毒性（NKCC）。在离体暴露于干扰素-γ（IFN-γ）后，NKCC针对肿瘤细胞靶标K562。使用四色流式细胞术检测NK（C56+ CD 3-）、CD 3 + CD 4+和CD 3 + CD 8+淋巴细胞的数量和百分比。