SSA - Elucidating the role of Endocytosis in Candida survival and proliferation in host cells

SSA - 阐明胞吞作用在宿主细胞中念珠菌存活和增殖中的作用

• 批准号: 1945527
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1945527
• 关键词: SSA; Elucidating; role; Endocytosis; Candida

Fungi such as Candida species are a major cause of hospital-acquired infections especially in elderly and immuno-compromised patients, and invasive candidiasis is associated with a high mortality rate. Central to Candida albicans virulence is the ability of cells to switch shape between round yeast and filamentous hyphal forms. This capacity is thought to allow the organism to disseminate effectively in blood (as yeast) and through tissues (with hyphae). There is increasing evidence implicating the endocytic pathway in the mechanism for bud to hyphal transition, and in the maintenance of polarised growth in hyphae. This project will allow unprecedented insight into machinery underlying C.albicans environmental fitness and interaction with host cells. Specifically, the student will-1. investigate the importance of the C.albicans endocytic pathway for survival and proliferation following uptake by macrophages2. develop tools to investigate surface levels of key proteins following macrophage uptake 3. use a zebrafish model system to determine whether endocytically-compromised Candida can proliferate and be disseminated in vivo Overall, the successful student will benefit from a multi-disciplinary project to be undertaken within internationally-recognized laboratories. They will gain expertise in in vitro and in vivo cell techniques as well as in a range of advanced microscopy approaches.

假丝酵母菌等真菌是医院获得性感染的主要原因，特别是在老年人和免疫功能低下的患者中，侵袭性念珠菌病与高死亡率有关。白念珠菌毒力的核心是细胞在圆形酵母和丝状菌丝之间转换形状的能力。这种能力被认为使有机体能够在血液中(作为酵母)和通过组织(通过菌丝)有效地传播。越来越多的证据表明，内吞途径参与了芽胞向菌丝过渡的机制，以及维持菌丝的极化生长。该项目将使人们能够前所未有地深入了解白念珠菌的环境适应性以及与宿主细胞的相互作用。具体地说，学生将-1。研究白念珠菌内吞途径对巨噬细胞摄取后生存和增殖的重要性2。开发研究巨噬细胞摄取后关键蛋白表面水平的工具3.使用斑马鱼模型系统来确定内生细胞受损的念珠菌是否可以在体内增殖和传播，成功的学生将受益于将在国际公认的实验室内开展的一个多学科项目。他们将在体外和体内细胞技术以及一系列先进的显微镜方法方面获得专业知识。