CYTOTOXIC T LYMPHOCYTE ACTIVATION IN VITRO AND IN VIVO

体外和体内细胞毒性 T 淋巴细胞激活

• 批准号: 6475691
• 负责人: MATTHEW Franklin MESCHER
• 金额: $ 21.09万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6475691
• 关键词: MHC class I antigen; T cell receptor; artificial membranes; cytokine; cytokine receptors; cytotoxic T lymphocyte; genetically modified animals; immunologic memory; interleukin 12; interleukin 2; laboratory mouse; leukocyte activation /transformation; passive immunization; receptor expression; tissue /cell culture

DESCRIPTION: (Adapted from the applicant's abstract) In this application, Dr. Mescher proposes to continue his studies identifying ligands that are essential for the activation of naive and memory cytotoxic T lymphocytes. Utilizing both his pioneering system of artificial surfaces for the delivery of defined signals and an adoptive transfer of CTLs from transgenic TCR mice, Dr. Mescher proposes to confirm much of his preliminary work identifying the key signals responsible for triggering CTL activation. This renewal application is expanded to include experiments to define the potential role that IL-12 plays in activating the naive CTL response as well as investigating a potential role for inflammatory cytokines in influencing memory cell function. Two specific aims, which are essentially identical to the specific aims of the original project, with the important addition of the adoptive transgenic mouse system are proposed.

描述：（改编自申请人的摘要）在此 申请后，梅舍尔博士建议继续他的研究，以确定 对于激活幼稚和记忆至关重要的配体 细胞毒性T淋巴细胞。利用他的开创性系统 用于传递定义信号和采用的人造表面 Mescher 博士建议从转基因 TCR 小鼠中转移 CTL 确认他的大部分前期工作，确定了关键信号 负责触发CTL激活。此续订申请是 扩展至包括确定 IL-12 潜在作用的实验 激活幼稚的 CTL 反应并调查 炎症细胞因子在影响记忆细胞中的潜在作用 功能。两个具体目标本质上是相同的 原始项目的具体目标，并添加了重要内容 提出了过继转基因小鼠系统。