Hydrolase discovery in the extremophile Galdieria

Galdieria是一种红色的植物，具有代谢的多样性，并在高酸性条件下（pH值低至0）和高于55 ℃的温度下（真核生物的理论极限）显示出巨大的生长能力。该属的成员显示出广泛的代谢谱，几乎可以在任何糖、糖醇或有机酸源上生长，并且能够进行光合自养和化能异养生长。Galdieria是一个代谢的主力。Galdieria的极端生活方式使其成为寻找具有工业生物技术（IB）应用特性的新物种的迷人生物。为了利用Galdieria用于IB领域，我们必须定义它所显示的代谢能力的多样性。在本项目的第一阶段，学生将进行IB相关酶的基因组发现。从那里，注释导致使用创新管道的代谢预测的描述，该管道利用基因组规模的代谢网络。在第二阶段，将对在极低pH值和高温下发挥作用的排泄酶进行信息描述。从分类酶的多样性中，同源性搜索将用于鉴定木聚糖酶和其他纤维素酶、蛋白酶和氧化酶。在第三阶段，学生将进行酶的特性分析，并开发出工业化前规模的工艺生产性能信息。根据定义，所有已鉴定的酶都必须在高温和极低的pH值下起作用。因此，鉴于Galdieria生活在真核生物的绝对极限，这些酶将是从真核生物中分离出的最耐热和耐酸的酶。

Galdieria is a red alga that exhibits metabolic versatility and displays enormous capacity to thriveat highly acidic conditions (down to pH 0) and temperatures above 55C, the theoretical limit ofeukaryotic life. Members in this genus display broad metabolic repertoires allowing vigorousgrowth on virtually any sugar, sugar-alcohol or organic-acid source and it is capable of bothphotoautotrophic and chemoheterotrophic growth. Galdieria is a metabolic workhorse. Theextremophile lifestyle of Galdieria makes it a fascinating organism to find those novel species withproperties that have industrial biotechnological (IB) applications. To exploit Galdieria for use in theIB sector, we must define the constellation of diversity in metabolic capacity it displays. In phase 1of this project, the student will perform phylogenomic discoveries of IB-relevant enzymes. Fromthere, annotation leads to a description of metabolic predictions using the innovative pipeline thatexploits genome-scale metabolic networks. In phase 2, informatic descriptions will be produced ofexcreted enzymes that can function under very low pH and elevated temperature. From the largediversity of classified enzymes, homology searches will be used to identify xylanases and othercellulases, proteases and oxidative enzymes. In phase 3, the student will perform enzymecharacterisation and develop information on process-production performance to a pre-industrialscale-up. By definition, all identified enzymes must function at high temperature and very low pH.Thus these will be the most heat- and acid-resistant enzymes ever isolated from a eukaryote,given that Galdieria lives at the absolute limits of eukaryotic life.