CD4 AND CORECEPTORS IN HIV-1/AIDS

HIV-1/AIDS 中的 CD4 和辅助受体

• 批准号: 6489237
• 负责人: David Kabat
• 金额: $ 27.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-15 至 2003-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6489237
• 关键词: AIDS; CD4 molecule; Cercopithecidae; HIV envelope protein gp120; Xenopus oocyte; animal genetic material tag; biological signal transduction; cell line; cellular pathology; genetic polymorphism; host organism interaction; human genetic material tag; human immunodeficiency virus 1; immunoprecipitation; molecular cloning; mutant; nucleic acid sequence; receptor binding; simian immunodeficiency virus; transfection /expression vector; virus infection mechanism; virus replication

DESCRIPTION: (Adapted from investigator's abstract) The goal of this project is to analyze quantitatively infections by primary patient (PR) and laboratory-adapted (LA) isolate viruses and to learn how differences in CD4 affinities affect cellular tropisms. The first of the investigator's three specific aims is to study quantitatively adsorption and penetration of PR and LA T cell-tropic HIV isolates into CD4- positive cells using cell clones with diverse amounts of CD4. The author proposes to study the kinetics of the steps of HIV attachment and to determine the order of CD4 involvement in each step. He will identify cellular factor(s) other than CD4 that limit attachment of LA viruses and their placement in the infection pathway. The second aim is to use molecularly cloned env genes of PR and LA viruses and of mutant viruses that have reduced CD4 affinities, and to identify features of env glycoproteins that control discrete steps of viral entry pathways. The third aim is to learn how absorptive properties of PR viruses change throughout disease progression. The fourth and last aim is to develop a novel system to study gp120- gp41- and CD4-dependent cell-cell fusion and to use it to study and possibly to clone cDNAs for cellular accessory factors.

描述：（改编自研究者摘要） 项目是定量分析主要患者（PR）的感染 和实验室适应（LA）分离病毒，并了解差异如何 在CD 4亲和力影响细胞向性。中的第一 研究者的三个具体目标是定量研究吸附 和PR和LA T细胞嗜性HIV分离株渗透到CD 4- 使用具有不同量的CD 4的细胞克隆检测阳性细胞。 的 作者建议研究HIV附着步骤的动力学， 以确定每个步骤中CD 4参与的顺序。 他将 鉴定除CD 4以外限制LA附着的细胞因子 病毒及其在感染途径中的位置。 第二个目的 是利用分子克隆的PR和LA病毒的env基因， CD 4亲和力降低的突变病毒，并鉴定其特征 控制病毒进入的不连续步骤 途径。 第三个目的是了解PR的吸收特性 病毒在疾病进展过程中不断变化。 第四个也是最后一个目标 目的是建立一个新的系统来研究gp 120-gp 41-和CD 4-依赖的 细胞-细胞融合，并利用它来研究和可能克隆cDNA， 细胞辅助因子。