Role of CIZ1 in X chromosome inactivation

CIZ1 在 X 染色体失活中的作用

• 批准号: 1949445
• 金额: --
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1949445
• 关键词: Role; CIZ1; chromosome; inactivation

X chromosome inactivation (XCI) serves as a model for establishment and maintenance of selective transcriptional silencing throughout the genome,through the action of polycomb repressive complexes (PRC) 1 and 2. The nuclear matrix (NM) protein Cip1-interacting zinc finger protein 1 (CIZ1) plays a role in DNA replication in association with cyclins, and is linked with adult and paediatric cancers. We recently showed that CIZ1 is located at the Xi in mouse and human female cells, and that absence of CIZ1 leads to highly penetrant female-specific lympho-proliferative disorder. The data argue for a role in maintenance of X chromosome inactivation through association with the nuclear matrix. This project will investigate the mechanism by which CIZ1 functions in the maintenance of epigenetic marks as cells pass through the cell cycle, and will use a range of molecular and cellular biology techniques including high resolution and live imaging.

X染色体失活(XCI)通过多梳抑制复合体(PRC)1和2的作用，在整个基因组中建立和维持选择性转录沉默的模型。核基质(NM)蛋白Cip1-相互作用的锌指蛋白1(CIZ1)在DNA复制中与细胞周期蛋白相关，并与成人和儿童癌症有关。我们最近发现，在小鼠和人类女性细胞中，CIZ1位于XI，缺失CIZ1会导致高度渗透性的女性特异性淋巴增殖性疾病。这些数据表明，X染色体失活的维持是通过与核基质的结合来实现的。该项目将研究CIZ1在细胞通过细胞周期时维持表观遗传标记的机制，并将使用一系列分子和细胞生物学技术，包括高分辨率和实时成像。