RYANODINE RECEPTOR REGULATION

描述(改编自申请人的摘要)： 拟议的研究是为了检验快速激活的假设 骨骼肌痉挛是通过改变磷酸化来调节的 兰尼定受体(RyR)肌浆网(SR)的状态 钙释放通道。在这个项目中，调查员将使用 反向磷酸化-碎裂-衍生化(BFD)和 碎片化Western印迹(FRAG-BLOT)法测定体内 不同RyR特定位点的磷酸化化学计量学研究 体内非激活肌肉中的亚型，以及强直性脊柱炎期间的亚型 刺激，以及在中度和重度疲劳时。大鼠伸肌 将比较长指肌(EDL)和蛙半腱肌 为了确定RyRs的磷酸化在包含以下内容的肌肉中是否有所不同 不同比例的RyR亚型。此外，选定的研究将 扩大到包括要测试的兔肉和鸡肉 哺乳动物和非哺乳动物物种之间的种间差异。

DESCRIPTION (Adapted from the Applicant's Abstract): The goal of the proposed studies is to test the hypothesis that activation of fast twitch skeletal muscles is regulated by altering the phosphorylation state of the ryanodine receptor (RyR) sarcoplasmic reticulum (SR) calcium release channels. In this project the investigator will use the back phosphorylation-fragmentation-derivatization (BFD) and fragmentation Western blot (frag-blot) methods to determine the in vivo phosphorylation stoichiometries of specific sites in the different RyR isoforms in vivo in nonactivated muscles, as well as during tetanic stimulation, and during moderate and severe fatigue. Rat extensor digitorum longus (EDL) and frog semitendinosus muscles will be compared o ascertain if phosphorylation of the RyRs differ in muscles containing different ratios of RyR isoforms. In addition, selected studies will be expanded to include rabbit and chicken muscles to test for interspecies variations between mammalian and nonmammalian species.