STRUCTURE DETERMINATION OF TUBULAR CRYSTALS

管状晶体的结构测定

• 批准号: 6525962
• 负责人: PETER Nigel UNWIN
• 金额: $ 23.15万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6525962
• 关键词: X ray crystallography; acetylcholine; chemical structure; cholinergic receptors; crystallization; membrane channels; method development; molecular shape; nanotechnology; structural biology

The proposed research is to develop the analysis of tubular structures into a standard electron crystallographic approach that can be used to (1) solve the structure of the nicotinic acetylcholine receptor at atomic resolution and (2) solve the three-dimensional structure from any tubular specimen in a semi-automated approach. To accomplish aim 1, improvements in the image processing routines will be made to extend resolution beyond the current 4.6A. Areas include more accurate measurement and correction of distortions, refined treatment to account for noise from overlapping Bessel terms, and corrections for Ewald sphere curvature, beam tilt and the contrast transfer function. Aim 2 will be achieved by analyzing tubular crystals of proteins being studied by other investigators using the methods established for the acetylcholine receptor after they have been automated. The rigorousness of the image processing and the broad applicability of the automated methodology will be determined.

建议的研究是为了发展管状结构的分析。 结构转化为标准电子结晶学方法，可用于 解决烟碱型乙酰胆碱受体的原子级结构问题 分辨率和(2)求解任意管状的三维结构 以半自动方法采集样本。 为实现目标1，将改进图像处理例程 将分辨率扩展到超过当前的4.6A。领域包括更准确的 扭曲的测量和纠正，精细的处理以说明 重叠贝塞尔项的噪声和对Ewald球面的修正 曲率、光束倾斜和对比度传递函数。 目标2将通过分析正在研究的蛋白质的管状晶体来实现 由其他研究人员使用为乙酰胆碱建立的方法 在它们被自动化之后，它们被接收。形象的严肃性 自动化方法的处理和广泛适用性将是 下定决心。