CORE--MORPHOLOGY FACILITY

核心——形态学设施

• 批准号: 6613349
• 负责人: DEBRA G. SILBERG
• 金额: $ 16.18万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6613349
• 关键词: antibody; biomedical facility; cell bank /registry; confocal scanning microscopy; cytology; digestive disorder; electron microscopy; human tissue; immunocytochemistry; in situ hybridization; liver disorder; morphology; tissue /cell preparation

The objective of the Morphology Core is to enhance the ability of the Center Investigators to perform digestive and liver research. The Morphology Core strives to provide high quality service and support to scientists with a commitment to teaching the techniques and providing the reagents necessary to perform morphologic analysis of gastrointestinal tract and liver tissues. This Core has grown substantially and has been extremely successful since July 1997, when it initiated services and provided reagents to Investigators of the Center for Molecular Studies in Digestive and Liver Disease. Dr. Silberg became the Director of the Morphology Core in 1998. The growth of the Core resulted in the hiring of Gary Swain, Ph.D. as the Technical Director. Drs. Silberg and Swain have been essential in expanding the scientific aspects of the Core by teaching techniques to Center Investigators and assisting in the development of protocols for new antibodies for immunohistochemistry. With the addition of two full time technicians, the Core has been able to provide quality services in a timely manner to the Center Investigators. The following description will outline the services provided by the Morphology Core. Morphologic data generated by some of the principle investigators will be presented as examples of the type and quality of work that can be expected. An important aspect of this core has been the collaboration and interactions that have occurred among its investigators. The Morphology Core is located on the 6th floor of the Clinical Research Building, in rooms adjacent to the scientific investigators in the Division of Gastroenterology, and easily accessible to all Center Investigators. Due to the close proximity of the Morphology Core to the user laboratories, it has become an important vehicle for Center Investigators, fellows, and technicians to collaborate and consult while performing immunohistochemistry or using the imaging equipment. Collaborations have been built through these interactions and we anticipate fostering future collaborations in this fashion. Importantly, the Molecular Biology Core is located across the hall from the Morphology Core, allowing for cohesive interactions in tissue procurement and analysis (e.g., Tissue bank, H&E sections and RNA extractions).

形态学核心的目标是增强中心研究人员进行消化和肝脏研究的能力。形态学核心致力于为科学家提供高质量的服务和支持，致力于教授胃肠道和肝脏组织形态学分析所需的技术并提供必要的试剂。自 1997 年 7 月开始为消化和肝脏疾病分子研究中心的研究人员提供服务并提供试剂以来，该核心已大幅发展并取得了巨大成功。 Silberg 博士于 1998 年成为形态学核心的总监。核心的发展导致聘用了 Gary Swain 博士。担任技术总监。博士。 Silberg 和 Swain 通过向中心研究人员教授技术并协助开发免疫组织化学新抗体方案，在扩展核心的科学方面发挥了重要作用。随着两名全职技术人员的加入，核心能够及时地为中心调查员提供优质的服务。以下描述将概述形态核心提供的服务。一些主要研究人员生成的形态学数据将作为预期工作类型和质量的示例提供。该核心的一个重要方面是其研究人员之间发生的合作和互动。形态学核心位于临床研究大楼 6 楼，房间内毗邻胃肠病学部门的科学研究人员，所有中心研究人员均可轻松进入。由于形态学核心距离用户实验室很近，它已成为中心研究人员、研究员和技术人员在进行免疫组织化学或使用成像设备时进行协作和咨询的重要工具。通过这些互动建立了合作，我们预计以这种方式促进未来的合作。重要的是，分子生物学核心位于形态学核心的对面，允许组织采购和分析（例如组织库、H&E 切片和 RNA 提取）中的内聚相互作用。