CORE--ANALYTICAL CYTOMETRY AND IMAGE ANALYSIS FACILITY

这一共享设施使中心的成员能够 高质量细胞计数和图像分析仪器和技术 服务仪器包括：（1）Coulter EPICS Elite flow （2）Coulter EPICS Profile II流式细胞仪; (3)Becton-Dickenson FACSCAN;（4）Meridian ACAS 570图像分析系统; (5)Meridian Insight激光扫描共聚焦显微镜这些文书 为研究人员提供细胞、颗粒和 组织样本研究者可以让机构工作人员分析样本，或 可以与技术专家合作分析样品和结果数据。 核心由黛布拉·拉斯金博士指导， EOHSI和CINJ的行政控制。咨询委员会召开会议 每半年或根据需要，与拉斯金博士和尤尔科博士（副主任， 设施核心）审查运营、使用优先政策和未来 布局 优先级由D博士确定。Laskin和E. Yurkow和基于自然 所需的项目和仪器。用户可以访问 一旦Yurkow博士或技术人员对他们进行培训，他们每天24小时使用仪器。 使用设施的费用是根据人员的时间（包括 设置）和用品的成本。EHS中心资助关键人员和 支付设备维护费用。对中心会员的收费是 比其他学术用户低50%至75%。 中心成员占所有用户的30-50%;大约25名EHS中心成员，来自 所有四个研究核心都使用了该设施举了几个例子 强调该基金对中心的重要性的项目 跨学科研究该基金最近扩大， 仪器和现有设备的大修。 未来的计划包括：（1）升级子午线ACAS系统的光学系统， （2）更换Coulter Profile II装置， （3）扩大现有的微型课程和实践教程 流式细胞术和图像分析方法。

This shared facility affords members of the Center access to high quality cytometry and image analysis instrumentation and technical services. Instrumentation includes: (1) Coulter EPICS Elite flow cytometer/cell sorter; (2) Coulter EPICS Profile II flow cytometer; (3) Becton-Dickenson FACSCAN; (4) Meridian ACAS 570 image analysis system; and (5) Meridian Insight laser scanning confocal microscope. These instruments provide investigators with multiparameter analyses of cells, particles and tissue samples. Investigators can have samples analyzed by Facility staff, or can work with technical experts to analyze samples and resultant data. The Core is directed by Dr. Debra Laskin and is under the shared administrative control of EOHSI and CINJ. An advisory committee meets biannually, or as needed, with Drs. Laskin and Yurkow (Associate Director of the Facility Core) to review operations, usage priority policies and future plans. Priorities are set by Drs. D. Laskin and E. Yurkow and are based on the nature of the project and instrumentation required. Users can have access to instruments 24 hr per day, once Dr. Yurkow or the technicians train them. Charges for the use of the facility are based upon personnel time (including setup) and the cost of supplies. EHS Center support funds key personnel and defrays the costs of equipment maintenance. Charges to center members are 50% to 75% lower than to other academic users. Center members comprise 30-50% of all users; about 25 EHS Center members, from all four Research Cores, have used the Facility. Several examples were given of projects which highlight the importance of this Facility to the Center s interdisciplinary research. The Facility was recently expanded with new instruments and with overhauls of existing units. Future plans include: (1) upgrade of the Meridian ACAS system optics and computer; (2) replacement of the Coulter Profile II unit with an new instrument; and (3) expansion of current mini-courses and hands-on tutorials in methods of flow cytometry and image analysis.