CORE--ELECTROPHYSIOLOGY AND XENOPUS OOCYTE LABORATORY

核心--电生理学和爪蟾卵细胞实验室

• 批准号: 6575674
• 负责人: DAVID C DAWSON
• 金额: $ 17.41万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6575674
• 关键词: Xenopus oocyte; animal genetic material tag; biomedical facility; electrophysiology; genetic manipulation

The objective of the Electrophysiology and Xenopus Oocyte is to make readily available to Toxicology Center investigators a variety of electrophysiological assays for ion transport and the Xenopus oocyte expression system for transporters and receptors. The rationale for establishing the core is that these techniques require specialized equipment and/or expertise that might not be available otherwise. The core will offer a broad range of assays ranging from flat-sheet, Ussing Chamber Studies to single channel recording and analysis. In addition the core will be equipped to harvest, defolliculate and inject, with RNA, Xenopus oocytes that can be used as an expression system for transporters or receptors. A primary aim of the Center for Membrane Toxicity Studies is to investigate the mechanism for the toxic effects of metals on membrane proteins, transporters and receptors. The Electrophysiology and Xenopus Oocyte Core will provide the equipment and expertise for Center investigators who wish to employ these important technologies in their research.

电生理学和爪蟾卵母细胞的目的是制造