CORE--MOLECULAR BIOLOGY FACILITY

描述（摘自申请）分子生物学核心设施由三个组件和Kasahara博士指导的子核心组成，Kasahara博士将监督基于病毒的基因传递系统的生成。分子生物学核心设施支持三大类活动。第一类主要的活动集中在开发特定的试剂来表达感兴趣的cdna。我们为非洲爪蟾卵母细胞的制备提供了广泛的资源，用于RNA表达研究和重组蛋白的细菌表达。我们已经添加了杆状病毒表达，并正在开发哺乳动物细胞系的诱导调节表达系统。此外，我们已经计划了一个新的亚核心，由新的联合主任指导，他将指导基于病毒的基因传递系统的发展。该组件还执行支持上面列出的不同表达系统所需的所有亚克隆活动。第二个组成部分是分子生物学指导和资源中心，介绍和指导没有或有限经验的研究人员利用分子生物学技术。这些服务包括在数据库中搜索序列同源性，设计引物用于PCR扩增和DNA测序。分子生物学核心设施的第三个组成部分是日常分子生物学研究所需的复杂设备的操作管理。

DESCRIPTION (Taken from the application) The Molecular Biology Core facility is composed of three components plus a sub-core directed by Dr. Kasahara, who will supervise generation of viral based gene delivery systems. The Molecular Biology Core facility supports three major classes of activities. The first major class of activity is focused on developing specific reagents to express cDNAs of interest. We are a campus wide resource for preparation of Xenopus laevis oocytes for RNA based expression studies and bacterial expression of recombinant proteins. We have added baculovirus expression and are developing inducible regulated expression systems for mammalian cell lines. In addition, we have planned a new sub-core directed by the new Co- Director, who will direct development of viral based gene delivery systems. This component also performs all the subcloning activities required to support the different expression systems listed above. The second component is a Molecular Biology Guidance and Resource Center, which introduces and guides investigators in utilizing molecular biological techniques who have no or limited experience. Included in these services are searching databases for sequence homologies and designing primers for PCR amplification and DNA sequencing. The third component of the Molecular Biology Core facility is operational management of sophisticated equipment needed in routine molecular biological studies.