N-glycosylation mechanism in insect cells.

描述（由申请人提供）：昆虫细胞被广泛认为是杆状病毒表达载体（bev）的宿主，它可以产生大量具有真核修饰的重组蛋白。昆虫细胞- bev系统已被用于生产许多不同的重组蛋白，促进了其结构、功能和在疾病中的作用的生物医学研究。许多公司正在使用昆虫细胞- bev系统来生产用于高通量功能筛选的重组蛋白，并开发疫苗、疗法和诊断试剂。在这些应用中使用昆虫细胞的优点包括易于扩大规模、成本较低以及不需要人类的外来因子。这些重要的研究活动需要深入了解昆虫细胞中蛋白质的生物合成和加工途径。然而，这并不是一个主要的研究课题。本文主要研究昆虫细胞蛋白n -糖基化途径。长期目标是提供昆虫细胞中这一重要代谢途径的明确和详细的观点，并利用这些知识开发具有更广泛重组糖蛋白加工能力的新的昆虫蛋白生产系统。

DESCRIPTION (provided by the applicant): Insect cells are widely known as hosts for baculovirus expression vectors (BEVs), which can produce large amounts of recombinant proteins with eukaryotic modifications. The insect cell-BEV system has been used to produce many different recombinant proteins, facilitating biomedical research on their structures, functions, and roles in disease. Many companies are using the insect cell-BEV system to produce recombinant proteins for high-throughput functional screens and to develop vaccines, therapeutics, and diagnostic reagents. The advantages of using insect cells for these applications include ease of scale-up, lower costs, and the absence of human adventitious agents. These important research activities require a dear understanding of protein biosynthesis and processing pathways in insect cells. However, this has not been a major topic of research. This proposal focuses on the insect cell protein N-glycosylation pathway. The long-term goals are to provide an unequivocal and detailed view of this important metabolic pathway in insect cells and to use this knowledge to develop new insect-based protein production systems with more extensive recombinant glycoprotein processing capabilities. The widespread use of insect cells as protein production systems for many important biomedical research projects is a strong justification for this research. Intelligent use of insect cells for recombinant glycoprotein production will require a better understanding of their N-glycosylation pathway. This understanding can direct innovative metabolic engineering efforts to produce new insect ceft-BEV systems with improved N-glycoprotein processing capabilities. Insect cells also occupy an important niche as models for fundamental research in the emerging field of glycobiology. The insect cell N-glycosylation pathway lies in-between the pathways of lower and higher eukaryotes in complexity. Thus, well-defined differences in insect protein glycosylation pathways could be exploited as specific targets to develop future insecticides. This could lead to better ways to control agriculturally and medically important insects and the diseases they help to spread, which would have a major impact on public health, worldwide.