CORE--NEUROPATHOLOGY FACILITY

神经病理学核心在我们的计划项目中发挥着核心作用。的 该核心的方法将是多学科的，使用传统的 神经病理学和最先进的分子神经生物学方法 以评估缺血性脑损伤的机制。核心工作人员 将进行定量神经病理学评估， 大鼠脑损伤的区域/细胞定位和严重程度， 小鼠局灶性脑缺血模型和小鼠和仔猪模型 心脏骤停/心肺复苏此外，核心将作为一个分子 北方免疫印迹集中使用神经病理学设施 印迹、原位杂交、组织化学和免疫细胞化学 用于定量测定缺血/再灌注诱导的 信号转导和细胞死亡途径的异常。的 由核心支持的调查人员有着悠久的历史， 在临床和实验神经病理学，神经解剖学， 和/或分子神经科学;因此，核心活动将支持 并协调分析实验材料产生的 计划项目。

The Neuropathology Core has a central role in our Program Project. The approach of this Core will be multidisciplinary, using conventional neuropathological and state-of-the-art molecular neurobiological methods to evaluate mechanisms of ischemic brain injury. The staff of the Core will conduct quantitative neuropathological assessments of the regional/cellular localization and severity of brain damage in rat and mouse models of focal cerebral ischemia and in mouse and piglet models of cardiac arrest/CPR. In addition, the Core will function as a molecular neuropathology facility for centralized use of immunoblotting, northern blotting, in situ hybridization, histochemistry, and immunocytochemistry for the quantitative determination of ischemia/reperfusion-induced abnormalities in signal transduction and cell death pathways. The investigators supported by the Core have a long-standing history of productivity in clinical and experimental neuropathology, neuroanatomy, and/or molecular neuroscience; therefore, the core activities will support and coordinate the analysis of experimental material generated in the Program Project.