CORE--NEUROBEHAVIORAL FACILITY

此核心的总体目标是提供一种设施行为 遗传、药物和外科操作对小鼠的影响 大鼠正在从中风或心脏骤停/心肺复苏中恢复。行为 检查是缺血后恢复期不可或缺的一部分， 与生理数据和组织学终点相关 动物。这是实验脑研究领域的一条新途径。 缺血和心脏骤停/心肺复苏，因为长期行为结果是 很少被检查过。大多数工作都强调组织学和形态学。 损伤(或恢复)的衡量标准。此外，在某些情况下，行为 变化可能是观察到的最显著的表型表达 突变的小鼠。在目标1和目标2中，西格玛的行为后果-- 在正常大鼠和缺血后大鼠以及在 PPBP治疗的小鼠以及神经细胞遗传缺陷的小鼠 一氧化氮合酶。在目标3和4中，我们将确定 实验性中风后的神经行为结果在以下方面更有利 雌性大鼠与雄性大鼠相比，转基因大鼠的中风损伤是否加剧 雌激素受体缺陷的小鼠(雌激素受体基因敲除)。 最后，在AIM中评估心脏骤停/心肺复苏后的认知功能障碍 5和3个转基因小鼠品系对神经元的细胞毒作用。 神经行为核心将利用从每个动物那里获得的动物 项目以获得对特定功能漏洞的新见解 脑缺血损伤的脑区及其可塑性潜能 感觉运动功能、记忆和简单认知任务的恢复。

The general objective of this Core is to provide a facility behavioral effects of genetic, pharmacological, and surgical manipulations in mice and rats tat are recovering from stroke or cardiac arrest/CPR. Behavioral examination is an integral part of the post-ischemic recovery period and is correlated with physiological data and histological end-points for each animal. This is a novel approach in the field of experimental cerebral ischemia and cardiac arrest/CPR because long term behavioral outcomes are rarely examined. Most work has emphasized histological and morphological measures of injury (or recovery). Further, in some cases, behavioral changes may be the most salient phenotypic expression observed among mutant mice. In Aims 1 and 2, the behavioral consequences of sigma- receptor activation are assessed in normal and post-ischemic rats and in PPBP treated mice, as well as mice genetically deficient in neuronal nitric oxide synthase. In Aims 3 and 4, we will determine if neurobehavioral outcomes after experimental stroke are more favorable in female versus male rats and if stroke injury is exacerbated in transgenic mice deficient in estrogen receptors (estrogen receptor knock-outs). Lastly, cognitive dysfunction after cardiac arrest/CPR is evaluated in Aim 5 and 3 transgenic mouse strains as a function of neuronal cytotoxicity. The Neurobehavioral Core will utilize the animals obtained from each project to gain new insight into the functional vulnerability of specific brain regions to ischemic injury and the potential for plasticity in recovery of sensory motor function, memory, and simple cognitive tasks.