CORE--NEUROBEHAVIORAL FACILITY

本核心的总体目标是提供一种设施行为 遗传、药理学和手术操作对小鼠的影响 并且大鼠达特正在从中风或心脏骤停/CPR中恢复。行为 检查是缺血后恢复期的组成部分， 与生理数据和组织学终点相关， 动物这是一种新的实验脑内 缺血和心脏骤停/CPR，因为长期行为结果 很少检查。大多数工作强调组织学和形态学 伤害（或恢复）的措施。此外，在某些情况下， 这些变化可能是观察到的最显著的表型表达， 突变小鼠在目标1和2中，sigma的行为后果- 受体活化在正常和缺血后大鼠中进行评估， PPBP处理的小鼠，以及神经元遗传缺陷的小鼠， 一氧化氮合酶在目标3和4中，我们将确定， 实验性中风后的神经行为结果更有利， 雌性大鼠与雄性大鼠相比， 雌激素受体缺陷小鼠（雌激素受体敲除）。 最后，在Aim中评估了心脏骤停/CPR后的认知功能障碍 5和3转基因小鼠品系作为神经元细胞毒性的函数。 神经行为核心将利用从每个实验室获得的动物， 项目，以获得新的洞察力的功能脆弱性的具体 脑区缺血性损伤和可塑性的潜力， 感觉运动功能、记忆和简单认知任务的恢复。

The general objective of this Core is to provide a facility behavioral effects of genetic, pharmacological, and surgical manipulations in mice and rats tat are recovering from stroke or cardiac arrest/CPR. Behavioral examination is an integral part of the post-ischemic recovery period and is correlated with physiological data and histological end-points for each animal. This is a novel approach in the field of experimental cerebral ischemia and cardiac arrest/CPR because long term behavioral outcomes are rarely examined. Most work has emphasized histological and morphological measures of injury (or recovery). Further, in some cases, behavioral changes may be the most salient phenotypic expression observed among mutant mice. In Aims 1 and 2, the behavioral consequences of sigma- receptor activation are assessed in normal and post-ischemic rats and in PPBP treated mice, as well as mice genetically deficient in neuronal nitric oxide synthase. In Aims 3 and 4, we will determine if neurobehavioral outcomes after experimental stroke are more favorable in female versus male rats and if stroke injury is exacerbated in transgenic mice deficient in estrogen receptors (estrogen receptor knock-outs). Lastly, cognitive dysfunction after cardiac arrest/CPR is evaluated in Aim 5 and 3 transgenic mouse strains as a function of neuronal cytotoxicity. The Neurobehavioral Core will utilize the animals obtained from each project to gain new insight into the functional vulnerability of specific brain regions to ischemic injury and the potential for plasticity in recovery of sensory motor function, memory, and simple cognitive tasks.