Role of Aquaporin in vocal fold epithelial water fluxes.

水通道蛋白在声带上皮水通量中的作用。

• 批准号: 6552551
• 负责人: DANIELLE N LODEWYCK
• 金额: $ 3.05万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6552551
• 关键词: animal tissue; biological fluid transport; immunocytochemistry; northern blottings; osmosis; predoctoral investigator; protein localization; respiratory epithelium; transmission electron microscopy; vocal cords; water channel; western blottings

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to better understand the cellular mechanisms, which regulate transepithelial water flux in the vocal fold. We hypothesize that aquaporin water channels, present in the plasma membrane of vocal fold epithelial cells, regulate osmostic transepithelial water fluxes of the vocal fold. If so, aquaporins may provide an intrinsic mechanism for regulation of vocal fold hydration and phonation. We will first demonstrate the presence of two aquaporin water channels in native vocal fold epithelial tissue via Western immunoblot analysis, followed by immunolocalization of the water channel proteins using immunogold labeling and transmission electron microscopy. The role of aquaporins in vocal fold transepithelial water fluxes will then be elucidated by imposition of osmotic gradients to the luminal epithelium. The dependence of aquaporin expression on the osmotic gradient will be demonstrated by Northern Blot analysis of mRNA expression of aquaporins and measuring associated transepithelial water fluxes. Data described here will contribute to knowledge of the flux of water across biological membranes as well as the cell biology of stratified squamous epithelial tissue, and may contribute to advances in clinical treatment of voice disorders.

描述（由申请人提供）：本提案的长期目标是更好地了解调节声带跨上皮水通量的细胞机制。我们推测，水通道蛋白水通道，存在于声带上皮细胞的质膜上，调节声带的跨上皮水通量。如果是这样的话，水通道蛋白可能提供了一个内在的机制，调节声带水化和发声。我们将首先证明存在两个水通道蛋白的水通道在天然声带上皮组织通过Western免疫印迹分析，然后通过免疫金标记和透射电子显微镜的水通道蛋白的免疫定位。水通道蛋白在声带经上皮水通量的作用，然后将阐明通过施加渗透梯度的腔上皮。水通道蛋白表达对渗透梯度的依赖性将通过水通道蛋白mRNA表达的北方印迹分析和测量相关的跨上皮水通量来证明。这里所描述的数据将有助于了解跨生物膜的水通量以及复层鳞状上皮组织的细胞生物学，并可能有助于嗓音疾病的临床治疗进展。