Developing Fibre Optic Raman Spectroscopy Probes for Cancer Diagnosis

开发用于癌症诊断的光纤拉曼光谱探针

• 批准号: 2074489
• 金额: --
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2074489
• 关键词: Developing; Fibre; Optic; Raman; Spectroscopy

Cancer will affect 50% of us in our lifetime and 25% of us will succumb to the disease. Early accurate detection and diagnosis is needed to provide the effective treatment regimes, however current methods are often insufficiently sensitive to early disease, or those that will progress to advanced cancers.We have pioneered the technique of Raman spectroscopy (RS) as a diagnostic tool for early identification of cancers. It utilises light to provide a real-time analysis or biochemical fingerprint of human cells, tissues, biofluids. It has been shown that RS is able to distinguish between pre-cancerous, cancerous and healthy tissue in the laboratory.Early prototype fibre probes for use in vivo have been developed and tested for use down an endoscope for hollow organ analysis, for use on the end of a smart needle and for near patient assessment of excised tissues. This project will further develop the Raman probes for point of care testing to maximise performance for specific applications of interest. This may involve coupling to complimentary imaging technologies.We plan to construct a smart needle to provide a real-time in vivo molecular analysis of tissue pathology at the tip of a needle. We will perform laboratory based ex vivo Raman analysis of tissue samples to optimise the specifications of the probe and make it fit for purpose. We will then undertake more advanced clinical validation studies and explore both spectral diagnostic and prognostic markers.To develop, build and test a smart needle device able to provide real-time, in vivo identification of malignancies using a fine fibre-optic Raman needle. The device will provide immediate results based on tissue biochemistry and allow for a rapid method for biopsy targeting or in vivo diagnosis.Aims:1) Build prototype with disposable needle tips able to measure required signatures in human tissues, to include sample volume and anatomical access requirements.2) Utilise device to provide signals from ex vivo tissue specimens.3) Build and test diagnostic models using these signals and compare results to gold standard histopathology.4) Evaluate probe performance in a clinical point of care setting

癌症将影响我们一生中50%的人，25%的人将死于这种疾病。早期准确的检测和诊断是提供有效的治疗方案所必需的，然而目前的方法往往对早期疾病或那些将发展为晚期癌症的疾病不够敏感。我们开创了拉曼光谱（RS）技术作为早期识别癌症的诊断工具。它利用光来提供人体细胞、组织、生物流体的实时分析或生化指纹。已经证明RS能够在实验室中区分癌前、癌性和健康组织。早期用于体内的原型光纤探针已经开发和测试，用于内窥镜下的中空器官分析，用于智能针头的末端，以及用于切除组织的近患者评估。该项目将进一步开发用于床旁检测的拉曼探针，以最大限度地提高感兴趣的特定应用的性能。这可能涉及耦合到互补的成像技术。我们计划构建一个智能针，以提供在针尖的组织病理学的实时体内分子分析。我们将对组织样本进行基于实验室的离体拉曼分析，以优化探针的规格并使其符合目的。然后，我们将开展更先进的临床验证研究，探索光谱诊断和预后标志物。开发、构建和测试一种智能针头设备，该设备能够使用精细光纤拉曼针头提供实时体内恶性肿瘤识别。该设备将提供基于组织生物化学的即时结果，并允许活检靶向或体内诊断的快速方法。目的：1）使用能够测量人体组织中所需特征的一次性针尖构建原型，以包括样本量和解剖入路要求。2）利用设备提供来自体外组织活检的信号。3）使用这些信号构建和测试诊断模型，并将结果与金标准组织病理学进行比较。4）在临床护理环境中评估探头性能