Periaqueductal Gray in Pain Modulatory Circuitry

疼痛调节回路中的导水管周围灰质

• 批准号: 6529300
• 负责人: CYNTHIA L RENN
• 金额: $ 2.41万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6529300
• 关键词: analgesia; antibody; behavior test; brain stem; central neural pathway /tract; chronic pain; fos protein; gene expression; hyperalgesia; immunocytochemistry; inflammation; laboratory rat; microinjections; neural information processing; neural transmission; neurochemistry; neurons; neurotransmitter receptor; neurotransmitters; norepinephrine; pain threshold; periaqueductal gray matter; predoctoral investigator; serotonin; stereotaxic techniques; stimulus /response

Despite recent advances in technology, achieving adequate pain management continues to challenge nurses and pain continues to be undertreated. The primary goal of this project is to increase understanding of the body's endogenous mechanisms of pain control, with anticipation of developing more efficacious treatment strategies. The specific aim of this proposal is to test the hypothesis that neurochemistry and neuronal activation within brainstem sites involved in descending modulation of nociception change over time following persistent inflammation and hyperalgesia and that the ventrolateral PAG (vlPAG) plays a key role in these changes. This hypothesis will be tested in a rat model of persistent inflammation. Two groups of rats, either with a chemical ablation of or an intact vlPAG, will be studied at three time points following inflammation; 0, 3, and 24 hours. It is anticipated that, using antibodies to neurochemicals and Fos protein, differences will be seen prior to and following inflammation. Moreover, the proposed experiments will enable the applicant to assess the role of the vlPAG in these changes. This study will provide the applicant with a variety of research techniques necessary to study endogenous pain mechanisms and will further the field in developing future treatments.

尽管技术取得了最新进展，但实现适当的疼痛管理仍在继续挑战护士，疼痛继续受到处理。该项目的主要目的是增加对人体内源性控制机制的理解，并期望制定更有效的治疗策略。该提案的具体目的是检验以下假设：在持续性炎症和超级过敏之后，脑干部位的神经化学和神经元激活随着时间的流逝而降低，并且腹侧PAG（VLPAG）在这些变化中起关键作用。该假设将在持续性炎症的大鼠模型中进行检验。两组大鼠在炎症后将在三个时间点进行研究，要么具有化学消融或完整的VLPAG。 0、3和24小时。预计，使用对神经化学物质和FOS蛋白的抗体，将在炎症之前和之后看到差异。此外，提出的实验将使申请人能够评估VLPAG在这些变化中的作用。这项研究将为申请人提供研究内源性疼痛机制所需的各种研究技术，并将进一步发展未来治疗的领域。