Tissue Homogenizaton by Pressure Cycling Technology

描述（由申请人提供）：使用压力循环的应用 技术（PCT）正在开发，以裂解细胞和均质化组织， 自动化生物样品处理。这项技术对于 在低通量和高通量应用中的自动化过程。第一阶段 研究表明，PCT非常有效地使动物和 植物组织样本PCT释放的核酸与 常规方法，适用于PCR和RT-PCR分析。细菌 样品可以在简单的裂解溶液中裂解， PCT适合PCR扩增。在这项II期研究中， 将用各种动物和植物样品评价均质化 不同程度的困难。PCT组织裂解的兼容性 将评估程序和一般核酸提取方法。 将开发多个平行样品处理。的最终目标 该项目旨在证明PCT裂解物在各种 使用核酸和蛋白质的生物测定。步骤 这种均质化过程的商业化将在 完成本II期研究。 拟定商业应用： 用于样品制备的压力循环技术（PCT）的发展将最终允许生物样品（例如细菌、动物和植物组织）的自动化均质化和裂解。PCT裂解物可用于在自动化过程中需要均质化生物样品的各种应用中。PCT均质技术的成功开发将有利于基因组学、医学、法医学、农业、制药、兽医和临床应用中基于分子的分析方法

DESCRIPTION (provided by applicant): An application using pressure cycling technology (PCT) is being developed to lyse cells and homogenize tissues for automated biological sample processing. This technology is feasible for automated processes in both low and high throughput applications. Our Phase I study demonstrated that PCT is very powerful to homogenize both animal and plant tissue samples. Nucleic acids released by PCT were comparable to conventional methods, and suitable for PCR and RT-PCR analysis. Bacteria samples could be lysed in simple lysis solution and crude lysate generated by PCT was suitable for PCR amplification. In this phase II study, tissue homogenization will be evaluated with a variety of animal and plant samples with different degrees of difficulties. Compatibility of the PCT tissue lysis procedure and the general nucleic acid extraction methods will be evaluated. Multiple parallel sample processing will be developed. The final objective of the project is to demonstrate utilities of PCT lysate in a variety of biological assays using nucleic acids and proteins. Steps towards commercialization of this homogenization process will be pursued upon completion of this Phase II study. PROPOSED COMMERCIAL APPLICATOIN: The development of the pressure cycling technology (PCT) for sample preparation will ultimately permit automated homogenization and lysis of biological samples, such as bacteria, animal and plant tissues. The PCT lysate may be utilized in a variety of applications that require homogenized biological samples in automated processes. The successful development of the PCT homogenization technology will benefit molecular based analytical procedures in genomics, medical, forensic, agricultural, pharmaceutical, veterinary and clinical applications