"The development of low toxicity cryoprotectants and cryopreservation techniques."

“低毒冷冻保护剂和冷冻保存技术的发展。”

• 批准号: 2097326
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2097326
• 关键词: development; low; toxicity; cryoprotectants; cryopreservation

Cells can be cooled to very low temperatures, halting their metabolism and allowing them to be stored indefinitely. However, this process results in a significant loss of viability and cell function due to freezing damage and cryoprotectant toxicity. This project will utilise a variety of techniques to minimise damage during the cryopreservation process. Intracellular ice formation is known to be highly lethal to cells. Developing effective cryoprotectants will be enhanced by an awareness of where ice is forming in a sample. As such, Raman microscopy will be used to image intracellular and extracellular ice formation during cell freezing. Traditional solvent cryoprotectants such as DMSO are damaging to cells and can cause adverse effects if those cells are transplanted into humans. A new non-toxic small molecular penetrating cryoprotectant will be synthesised and tested, with the aim of reducing the amount of solvent needed for cryopreservation. Raman microscopy will be used to map the intracellular presence of this cryoprotectant and determine its relationship to ice formation.

细胞可以被冷却到非常低的温度，停止它们的新陈代谢并允许它们无限期地储存。然而，由于冷冻损伤和冷冻保护剂毒性，该过程会导致活力和细胞功能的显着丧失。该项目将利用多种技术来最大程度地减少冷冻保存过程中的损害。已知细胞内冰的形成对细胞具有高度致命性。了解样品中结冰的位置将有助于开发有效的冷冻保护剂。因此，拉曼显微镜将用于对细胞冷冻过程中细胞内和细胞外冰的形成进行成像。传统的溶剂型冷冻保护剂（例如 DMSO）会损害细胞，如果这些细胞移植到人体中，可能会产生不利影响。将合成并测试一种新型无毒小分子渗透性冷冻保护剂，旨在减少冷冻保存所需的溶剂量。拉曼显微镜将用于绘制这种冷冻保护剂的细胞内存在情况并确定其与冰形成的关系。

项目成果

Red Blood Cell Cryopreservation with Minimal Post-Thaw Lysis Enabled by a Synergistic Combination of a Cryoprotecting Polyampholyte with DMSO/Trehalose.

• DOI: 10.1021/acs.biomac.1c00599
• 发表时间: 2022-02-14
• 期刊: Biomacromolecules
• 影响因子: 6.2
• 作者: Murray A;Congdon TR;Tomás RMF;Kilbride P;Gibson MI
• 通讯作者: Gibson MI