MOUSE SYNAPTIC VESICULAR MONAMINE TRANSPORTER

小鼠突触囊泡单胺转运蛋白

• 批准号: 6103871
• 负责人: George Richard Uhl
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6103871
• 关键词: amphetamines; animal genetic material tag; complementary DNA; dopamine; dopamine transporter; embryonic stem cell; gene deletion mutation; genetic regulatory element; laboratory mouse; methylphenyltetrahydropyridine; molecular cloning; neurotoxicology; neurotoxins; neurotransmitter transport; nucleic acid sequence; synaptic vesicles; transcription factor; transfection /expression vector

Activities of the principal brain vesicular monamine transporter, VMAT2, are key to understanding the cellular compartmentalization of monoamines that may play a key role in modulating the actions and neurotoxicities induced by amphetamine and by each of the toxins that selectively kills dopaminergic neurons to provide the best current models of Parkinson's disease. In this FY, workers in this Branch described the properties of knockout mice with deletions of the VMAT2 gene, as well as the structure of this gene itself in mice and humans. VMAT2 deficient heterozygote mice show selective alterations in their responses to cocaine and amphetamine, as well as differences in MPTP toxicities. These mice substantially enhance our understanding of mechanisms of psychostimulant and dopaminergic neurotoxin action.

主脑泡状单胺的活性 转运蛋白VMAT2是理解细胞 单胺的区隔作用，这可能在 调节苯丙胺的行为和神经毒性 通过每种选择性地杀死多巴胺能神经元的毒素 提供目前最好的帕金森氏病模型。在这 仅供参考，该分支机构的工作人员描述了击倒的属性 VMAT2基因缺失的小鼠，以及VMAT2基因的结构 这种基因本身就存在于老鼠和人类体内。VMAT2缺陷型杂合子 小鼠对可卡因和可卡因的反应出现选择性改变 苯丙胺，以及MPTP毒性的差异。这些老鼠 大大提高了我们对这一机制的理解 精神刺激剂和多巴胺能神经毒素作用。