EBP1 CONTROL OF PROSTATE CANCER CELL GROWTH

EBP1 控制前列腺癌细胞生长

• 批准号: 6498063
• 负责人: ANNE W. HAMBURGER
• 金额: $ 11.14万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6498063
• 关键词: androgen inhibitor; androgen receptor; genetic promoter element; genetic transcription; heregulin; intracellular transport; luciferin monooxygenase; neoplastic growth; oncoproteins; prostate neoplasms; protein protein interaction; protein transport; protein tyrosine kinase; receptor binding; recombinant proteins; reporter genes; tissue /cell culture; transport proteins

DESCRIPTION (adapted from the applicant's abstract): The applicant's group has recently cloned a new protein, Ebp1, which interacts with the cytoplasmic domain of the heregulin/neuregulin receptor, ErbB3. Ectopic expression of Ebp1 inhibits androgen dependent activation of androgen responsive reporter constructs. The purpose of this proposal is to understand the mechanism by which Ebp1 inhibits androgen receptor (AR) mediated transcription. The first specific aim will: (1) determine the ability of Ebp1 to modulate AR-mediated transcription from different wild type and natural androgen-responsive promoters and from endogenous genes; (2) determine the ability of Ebp1 to inhibit the activity of AR transcriptional coactivators; (3) determine if Ebp1 can inhibit ErbB2 induction of AR-mediated transactivation. In the second aim the applicant will: (1) confirm and extend the observation that AR and Ebp1 physically associate and (2) determine the regions of AR and Ebp1 needed for protein-protein binding. Deletion constructs generated will be used to determine if the inhibitory effects of Ebp1 on AR-mediated transcription and cell growth are related to the ability of this protein to bind AR.

描述（改编自申请人的摘要）：申请人所在的小组有