Tyrosine Nitration of Prostacyclin Synthase
Tyrosine Nitration of Prostacyclin Synthase
批准号:
6538025
负责人:
Aimee Leigh Landar
金额:
$4.62万
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
未结题
起止时间:
2002-05-01 至
中文摘要
描述(申请人?促炎症反应是一个重要的过程,
动脉粥样硬化病变和其他病理生理学的发展
条件在动脉粥样硬化病变中,过氧亚硝酸盐,由
一氧化氮与超氧阴离子、硝酸盐蛋白质对酪氨酸的反应
残基这个项目有两个目标,将测试假设,
过氧亚硝酸盐通过硝化激活内皮中的促炎状态,
使前列环素合酶失活。内皮细胞中的这种关键酶
在其活性位点附近的酪氨酸430处易于过氧亚硝酸根硝化。
具体目标1是利用噬菌体展示的强大方法,
链抗体,开发新的抗体,
硝基酪氨酸表位,以及前列环素中的硝化酪氨酸430
合成酶具体目标2是评估剪切应力是否也硝酸盐,
使前列环素合酶失活,导致促炎性细胞因子增加,
前列环素前体,PGH2。.
英文摘要
DESCRIPTION (Applicant?s abstract):Proinflammation is an important process in
the development of atherosclerotic lesions and other pathophysiological
conditions. In atherosclerotic lesions, peroxynitrite, which is formed by the
reaction of nitric oxide and superoxide, nitrates proteins on tyrosine
residues. This project has two aims that will test the hypothesis that
peroxynitrite activates a proinflammatory state in endothelium by nitrating and
inactivating prostacyclin synthase. This key enzyme in endothelium is unusually
susceptible to peroxynitrite nitration at tyrosine 430 near its active site.
Specific aim 1 is to use the powerful methodology of phage display of single
chain antibodies to develop new antibodies that recognize the small
nitrotyrosine epitope, as well as nitrated tyrosine 430 in prostacyclin
synthase. Specific aim 2 is to assess whether shear stress also nitrates and
inactivates prostacyclin synthase, resulting in the increase of proinflammatory
prostacyclin precursor, PGH2. .
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会议论文
The role of the mitochondrial protein thiol modification in endothelial dysfuncti
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批准号:8045419
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项目类别:
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资助金额:$36.63万
-
财政年份:2010
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负责人:Aimee Leigh Landar
-
依托单位:
The role of mitochondrial protein thiol modification in endothelial dysfunction
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批准号:8432823
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项目类别:
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资助金额:$34.52万
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财政年份:2010
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负责人:Aimee Leigh Landar
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依托单位:
The role of mitochondrial protein thiol modification in endothelial dysfunction
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批准号:8627195
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项目类别:
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资助金额:$35.53万
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财政年份:2010
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负责人:Aimee Leigh Landar
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依托单位:
The role of the mitochondrial protein thiol modification in endothelial dysfuncti
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批准号:7887903
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项目类别:
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资助金额:$36.63万
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财政年份:2010
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负责人:Aimee Leigh Landar
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依托单位:
The role of mitochondrial protein thiol modification in endothelial dysfunction
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批准号:8220867
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项目类别:
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资助金额:$36.26万
-
财政年份:2010
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负责人:Aimee Leigh Landar
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依托单位:
Tyrosine Nitration of Prostacyclin Synthase
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批准号:6339891
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项目类别:
-
资助金额:$4.02万
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财政年份:2001
-
负责人:Aimee Leigh Landar
-
依托单位:
海外基金