IDENTIFICATION OF ECTODERM DETERMINANTS IN XENOPUS

非洲爪蟾外胚层决定因素的鉴定

• 批准号: 6530581
• 负责人: DOUGLAS W HOUSTON
• 金额: $ 4.37万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6530581
• 关键词: Xenopus; developmental genetics; ectoderm; embryogenesis; expression cloning; genetic library; genetic markers; germ layers; postdoctoral investigator; transcription factor

DESCRIPTION (provided by applicant) The goal of this research is to identify the molecular basis of primary germ layer formation in vertebrate embryos. This fundamental cell fate decision is only partially understood. Recently, it has been discovered that in Xenopus, mesoderm and endoderm cell fates are specified by maternal transcription factors. In this study, we focus on what determines the ectoderm germ layer. We have identified ectoderm-inducing activities in pools of maternal cDNAs, and we will use expression cloning to identify these genes. Subsequently we will characterize the expression of these genes and determine their roles in development through loss-of-function experiments. Understanding the formation of the ectoderm will be a major step forward in understanding cell fate determination in early development.

描述（由申请人提供）本研究的目的是确定脊椎动物胚胎初级胚层形成的分子基础。这个基本的细胞命运决定只是部分地被理解。近年来，人们发现非洲爪蟾的中胚层和内胚层细胞命运是由母体转录因子决定的。在这项研究中，我们重点研究是什么决定了外胚层的胚层。我们已经在母体cdna中鉴定了外胚层诱导活性，我们将使用表达克隆来鉴定这些基因。随后，我们将表征这些基因的表达，并通过功能丧失实验确定它们在发育中的作用。了解外胚层的形成将是了解早期发育中细胞命运决定的重要一步。