Arrays to Measure Endocrine Disruption in Fish

测量鱼类内分泌干扰的阵列

• 批准号: 6548003
• 负责人: PATRICK M LARKIN
• 金额: $ 17.13万
• 依托单位: AQUAGENE, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6548003
• 关键词: biosensor device; endocrine gland /system; environmental exposure; environmental toxicology; fish; fresh water environment; gene environment interaction; gene expression; hazardous substances; hormone receptor; hormone regulation /control mechanism; microarray technology; technology /technique development; water pollution

DESCRIPTION (provided by applicant): Endocrine disrupting compounds (EDCs) in the environment can cause a variety of physiological problems in animals and humans. Thus, measurement and analysis of EDCs in the environment is very important to the Environmental Protection Agency (EPA) in its Superfund monitoring activities. The investigators have developed a prototype macroarray that works exceptionally well with sheepshead minnow to predict estrogenic outcomes in laboratory-exposed fish. They seek to apply this technology to produce a macroarray membrane (or chip) for largemouth bass that would contain cDNAs for many of the major hormone receptors, many of their downstream-regulated genes and additional genes of toxicological importance. Gene arrays are powerful tools for identifying molecular changes in animals. Endocrine disrupting compounds act at the gene level to induce or repress gene expression through both receptor-mediated and non-receptor- mediated pathways. Based on preliminary data, the investigators expect to be able to identify gene expression patterns related to specific types of contaminants present in mixtures. They seek funding to prepare the arrays, test them under laboratory-exposed conditions and to test them at Superfund sites.

描述（由申请人提供）：环境中的内分泌干扰化合物（EDC）可引起动物和人类的多种生理问题。 因此，环境中 EDC 的测量和分析对于环境保护局 (EPA) 的超级基金监测活动非常重要。 研究人员开发了一种原型宏阵列，它对羊头鲦鱼效果非常好，可以预测实验室暴露的鱼的雌激素结果。 他们寻求应用这项技术来生产大口黑鲈的宏阵列膜（或芯片），其中包含许多主要激素受体的 cDNA、许多下游调节基因以及具有毒理学重要性的其他基因。 基因阵列是识别动物分子变化的强大工具。 内分泌干​​扰化合物在基因水平上起作用，通过受体介导和非受体介导的途径诱导或抑制基因表达。 根据初步数据，研究人员希望能够识别与混合物中存在的特定类型污染物相关的基因表达模式。 他们寻求资金来准备阵列，在实验室暴露的条件下对其进行测试，并在超级基金站点进行测试。