Enzymatic Tools for Degrading Tissue and Preserving RNA
Enzymatic Tools for Degrading Tissue and Preserving RNA
批准号:
6548165
负责人:
GARY J LATHAM
金额:
$16.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-09-27 至 2003-08-31
中文摘要
描述(由申请人提供):
英文摘要
DESCRIPTION (provided by applicant):
Intact RNA is an obligate starting material for expression profiling
technologies that can link specific molecular events with disease phenotypes.
RNA isolations from tissue currently require invasive mechanical force to
disrupt the cellular architecture. Such mechanical approaches are tedious and
potentially inefficient. They also expose the operator to possible biohazards.
As a result, the development of a "closed system" for RNA isolation is needed
to enable the high-throughput recovery of RNA from tissues such as biopsies
using a safeguarded platform that can process dozens or hundreds of tissues at
once. We proposed to achieve this goal through the use of "MELT"
(Multi-Enzymatic Liquefaction of Tissue) technology. This technology will
harness the degrading power of enzymes that have evolved together as a
biological tool set for the decomposition of complex food sources. We refer to
this tool set as an "enzyme community." Since some enzyme communities (e.g.,
the secreted enzymes of bacterial pathogens) mimic the spectrum of activities
needed to digest tissue, these collections are an ideal repository for
tissue-degrading enzymes. We propose to characterize and blend enzyme
activities to create a MELT reagent that can rapidly degrade tissues, "freeze"
mRNA expression profiles, and stabilize the RNA for downstream analyses.
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海外基金