A High Yield Affinity Cell Separation Technology

本项目的长期目标是开发一种特别高产、基于亲和性的细胞分离技术。该技术具有几个有价值的潜在临床应用。我们将集中于从血液中分离罕见的循环肿瘤细胞。这项技术将有助于开发一种可重复的检测方法，用于在转移发生之前和手术可以治愈的早期检测隐匿性癌症。用抗体将细胞捕获到固体表面的限制因素是细胞与其所要连接的表面之间的附着位点的数量。增加细胞和固相之间的连接数量将预期增加附着强度和所得细胞产率。我们的技术开发计划侧重于一种方法，以增加连接链接的数量。在这第一阶段的努力，我们将开发两种新的配体，表现出极高的附着密度的固体表面。我们将这些配体结合到淋巴细胞特异性抗体上，并测试它们的有效性。具体来说，我们将测量这些配体介导与固体表面的强有力附着的能力，并将其与传统的、现有的亲和细胞分离技术进行比较

DESCRIPTION (provided by applicant) The long-term objective of this project is the development of an exceptionally high yield, affinity-based cell separation technology. The technology has several valuable potential clinical applications. We will focus on the isolation of rare circulating neoplastic cells from blood. This technology will facilitate the development of a reproducible test for the detection of occult cancers at an early point in time, before metastases occur and when surgery could be curative.The limiting factor in capturing cells to a solid surface with antibodies is the number of attachment sites between a cell and the surface to which it is to be tethered. Increasing the number of links between the cell and the solid phase will be expected to increase the strength of attachment and the resulting cell yield. Our technology development program focuses on a method to logarithmically increase the number of attachment links. In this Phase I effort, we will develop two novel ligands that demonstrate extremely high densities of attachment to solid surfaces. We will conjugate these ligands to lymphocyte-specific antibodies and test their effectiveness. Specifically, we will measure the ability of these ligands to mediate a powerful attachment to the solid surface and compare them to conventional, existing affinity cell separation technologies