Pneumatic Focusing Breath Analysis of Test Animals

描述(申请人摘要)：气动聚焦气相色谱 (PFGC)是一种新的、全自动化的痕量气体分析技术，在 波特兰州立大学环境空气污染分析。PSU已申请 申请专利，并将授予VOC Technologies独家许可。这个 样机已运行1年多，耗时15,000人次 相对挥发性有机物中&gt；30种挥发性有机物的色谱分析 俄勒冈州波特兰的清新空气。这项技术非常适合呼吸。 分析，并将适用于全自动、连续的测量 实验室测试生物体呼吸中存在的微量代谢物。如果证明 在原理测试之后，原型PFGC将被用于正在进行的 俄勒冈健康科学大学对正常和低氧大鼠的研究。这 由美国国立卫生研究院赞助的一项研究探讨了缺氧缺血在发病机制中的作用 通过评估两种极有希望的围产期脑损伤模型 围产期缺氧缺血性脑白质损伤大鼠和小鼠。这个 在这些非常小的新生啮齿动物中发生的代谢障碍是 在成年动物身上很难用标准方法进行评估。成功 对这一原型的测试将导致商业仪器的更多 代谢率和代谢率的实验室研究中广泛的、协作的贝塔测试 疾病计划在第二阶段。

DESCRIPTION (Applicant's abstract): Pneumatic focusing gas chromatography (PFGC) is a new, fully-automated technique for trace-gas analysis developed at Portland State University for ambient air pollution analysis. PSU has applied for a patent and will grant VOC Technologies an exclusive license. The prototype instrument has operated for more than 1 year, taking >15,000 chromatograms quantifying >30 volatile organic compounds in the relatively clean air of Portland Oregon. This technology is ideally suited for breath analysis and will be adapted for fully automated, continuous measurements of trace metabolites present in the breath of laboratory test organisms. If proof of principle testing, the prototype PFGC will then be employed in an ongoing study of normal and hypoxic rats at the Oregon Health Sciences University. This NIH-sponsored study explores the role of hypoxia-ischemia in the pathogenesis of perinatal brain injury by evaluating two highly promising models of perinatal hypoxia-ischemic cerebral white matter injury rats and mice. The metabolic disturbances that occur in these very small neonatal rodents are difficult to assess by standard methodologies in adult animals. Successful testing of this prototype will lead to a commercial instrument for more widespread, collaborative beta testing in laboratory studies of metabolism and disease planned for Phase 2.