CORE--STRUCTURAL ANALYSIS

描述(由申请人提供)： 核心B为PPG调查人员提供了关键资源，并已被大量使用 在过去的10年里。预计随着PPG的支持，使用量将会增加 研究变得更加专注于基因表达和基因 在发育中的肺的调节。核心将提供：1)科学 关于选择适当技术和咨询的专门知识和咨询 试剂、对照设计和结果解释；2)培训 PPG人员学习他们工作所需的技术，包括免疫染色，在 原位杂交、显微镜和图像分析；3)关键设备和资金 用于设备维护；4)本程序中常用的用品 (幻灯片、石蜡等)；以及5)一名熟练的技术人员来监督设备 教书育人，规范新方法，更新技术途径。 免疫组织化学和原位杂交研究包括大部分 在核心实验室中完成的工作，尽管核心人员拥有许多 如有需要，可采用其他工艺。已经为以下方面准备了书面技术协议 这些技术和在线供PPG调查人员使用。所有关键设备都是 可用且功能齐全。核心有一个莱茨显微镜的异常 高品质，适用于明场、相场、暗场和 荧光显微镜。这台显微镜配备了一个摄像头，摄像头上有 输入到由OpenLab软件编程的Macintosh G3计算机以获取图像 分析，包括高级测量、指定计数和荧光 放大。

DESCRIPTION (provided by applicant): Core B provides a key resource for PPG investigators and has been heavily used in the past 10 years. It is anticipated that use will increase as PPGsupported research becomes more sharply focused on gene expression and gene regulation in the developing lung. The Core will provide: 1) scientific expertise and consultation as to selection of appropriate techniques and reagents, design of controls, and interpretation of findings; 2) training for PPG personnel in techniques needed for their work including immunostaining, in situ hybridization, microscopy, and image analysis; 3) key equipment and funds for equipment maintenance; 4) supplies commonly used in these procedures (slides, paraffin, etc.); and 5) a skilled technician to supervise equipment and teach personnel, standardize new methods, and update technical approaches. Immunohistochemical and in situ hybridization studies comprise much of the work done in the Core Lab, although Core personnel have experience with many other technics if needed. Written technical protocols have been prepared for these technics and are online for PPG investigators. All key equipment is available and functional. The Core has a Leitz microscope of exceptionally high quality that is equipped for brightfield, phase, darkfield, and fluorescence microscopy. The microscope is equipped with a video camera with input to a Macintosh G3 computer programmed by OpenLab software for image analysis including advanced measurements, specified counting, and fluorescence amplification.