Core--Gene expression profiling

核心--基因表达谱

• 批准号: 6602450
• 负责人: Gary D Swergold
• 金额: $ 26.82万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6602450
• 关键词: atherosclerosis; biomedical facility; gene expression; genetic library; genetic mapping; microarray technology

The Gene Expression Profiling core will carry out studies of differences in gene expression in mouse tissues. This will primarily involve the use of cDNA or EST libraries arrayed on glass slides. These will be probed with RNA (reverse transcribed into fluorescently labeled cDNA) prepared from the relevant tissues (control condition and perturbed condition). These studies will employ a Genetic Microsystems model GMS 417 Arrayer, model GMS418 dual wavelength laser confocal Array Scanner and Imaging Research Array Vision software. Arrays will consist of difference libraries or mouse Unigene sets. These studies will aid in the identification of atherosclerosis susceptibility genes (Project 1) and in the discovery of novel genes involved in the macrophage response to atherosclerosis (Projects 2-4).

基因表达谱核心将进行小鼠组织中基因表达差异的研究。这将主要涉及使用排列在载玻片上的cDNA或EST文库。这些将用从相关组织（对照条件和扰动条件）制备的RNA（逆转录为荧光标记的cDNA）进行探测。这些研究将使用Genetic Microsystems型号GMS 417阵列仪、型号GMS 418双波长激光共聚焦阵列扫描仪和成像研究阵列视觉软件。阵列将由差异文库或小鼠Unigene组组成。这些研究将有助于鉴定动脉粥样硬化易感基因（项目1）和发现参与巨噬细胞对动脉粥样硬化反应的新基因（项目2-4）。