Elucidating the honey bee immune response and pathogen transmission at varroa mite feeding sites

阐明瓦螨进食地点的蜜蜂免疫反应和病原体传播

• 批准号: 2104320
• 金额: --
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2104320
• 关键词: Elucidating; honey; bee; immune; response

Honey bees (Apis mellifera) are critically important to global food production by virtue of their pollination services. Worryingly, there is a well-recognised honey bee health crisis threatening bee populations and, thereby, global food security. Of particular note has been the spread of the external parasitic mite Varroa destructor from the Asian honey bee (Apis cerana) to a near global presence on the Western honey bee (A. mellifera) over the past 50 years. More recently, it has been recognized that the high lethality of Varroa infestation in a honey bee colony is not due to Varroa in isolation but due to its interaction with the deformed wing virus (DWV).The maternal Varroa creates a bite site on the immature bee that does not heal, thus, allowing her offspring to feed from the communal feeding site for the rest of their development. During this biting and feeding the Varroa spit salivary bioactive compounds to the bite site preventing it from healing over, modulating the honey bee's immune system and also transmitting deformed wing virus. The aim of the project is to elucidate the interaction of the Varroa-DWV-honey bee within the vicinity of the bite site and disseminating from there.The project offers the student much scope for developing its direction as it progresses. A full programme of generic skill training is available. The project will provide training in insect immunology, acarology, modern molecular biology techniques (RNAseq, bioinformatics, gene knockdown, qPCR, in situ hybridization), virology, histology and basic bee husbandry.

蜜蜂（Apis mellifera）凭借其授粉服务对全球粮食生产至关重要。令人担忧的是，有一个公认的蜜蜂健康危机威胁着蜜蜂种群，从而威胁着全球粮食安全。特别值得注意的是，在过去的50年里，外来寄生性螨虫瓦螨从亚洲蜜蜂（Apis cerana）传播到几乎全球的西方蜜蜂（a . mellifera）。最近，人们已经认识到，蜂群中瓦螨的高致死率不是由于孤立的瓦螨，而是由于它与变形翼病毒（DWV）的相互作用。母瓦罗亚在未成熟的蜜蜂上制造了一个不会愈合的咬痕，这样，她的后代就可以在他们发育的其余时间里从公共喂食点喂养。在叮咬过程中，瓦罗阿蜂会将唾液中的生物活性化合物喂到叮咬部位，防止伤口愈合，调节蜜蜂的免疫系统，并传播变形的翅膀病毒。该项目的目的是阐明瓦罗亚- dwv蜜蜂在叮咬地点附近的相互作用，并从那里传播。这个项目为学生提供了很大的发展方向的空间。提供全面的一般技能培训方案。该项目将提供昆虫免疫学、昆虫学、现代分子生物学技术（RNAseq、生物信息学、基因敲低、qPCR、原位杂交）、病毒学、组织学和基础养蜂学方面的培训。